Zhang Zhen-Shan, Liu Yu-Lan, Che Li-Ming
College of Food Science and Technology, Henan University of Technology.
Department of Chemical and Biochemical Engineering, Xiamen University.
J Oleo Sci. 2018 Mar 1;67(3):255-263. doi: 10.5650/jos.ess17153. Epub 2018 Feb 19.
Supercritical carbon dioxide extraction (SC-CO) technology was used to extract oil from Eucommia ulmoides seed. The optimum conditions and significant parameters in SC-CO were obtained using response surface methodology (RSM). The qualities of the extracted oil were evaluated by physicochemical properties, fatty acid composition, vitamin E composition. It was found that the optimum extraction parameters were at pressure of 37 MPa, temperature of 40°C, extraction time of 125 min and CO flow rate of 2.6 SL/min. Pressure, temperature and time were identified as significant parameter effecting on extraction yield. The importance of evaluated parameters decreased in the order of pressure > extraction time > temperature > CO flow rate. GC analysis indicated that E. ulmoides seed oil contained about 61% of linolenic acid and its fatty acid composition was similar with that of flaxseed oil and perilla oil. The content and composition of vitamin E was determined using HPLC. The E. ulmoides seed oil was rich in vitamin E (190.72 mg/100 g), the predominant vitamin E isomers were γ- tocopherol and δ- tocopherol, which accounted for 70.87% and 24.81% of the total vitamin E, respectively. The high yield and good physicochemical properties of extracted oil support the notion that SC-CO technology is an effective technique for extracting oil from E. ulmoides seed.
采用超临界二氧化碳萃取（SC-CO）技术从杜仲种子中提取油脂。运用响应面法（RSM）获得了SC-CO萃取的最佳条件和显著参数。通过物理化学性质、脂肪酸组成、维生素E组成对所提取油脂的品质进行评价。结果发现，最佳萃取参数为压力37MPa、温度40℃、萃取时间125min、CO流量2.6SL/min。压力、温度和时间被确定为影响萃取率的显著参数。各评价参数的重要性排序为压力＞萃取时间＞温度＞CO流量。气相色谱分析表明，杜仲种子油中含有约61%的亚麻酸，其脂肪酸组成与亚麻籽油和紫苏油相似。采用高效液相色谱法测定了维生素E的含量和组成。杜仲种子油富含维生素E（190.72mg/100g），主要的维生素E异构体为γ-生育酚和δ-生育酚，分别占总维生素E的70.87%和24.81%。所提取油脂的高产率和良好的物理化学性质支持了SC-CO技术是从杜仲种子中提取油脂的有效技术这一观点。
