Palacios J M, Mengod G, Vilaró M T, Ramm P
Preclinical Research, Sandoz Pharma Ltd, Basel, Switzerland.
J Chem Neuroanat. 1991 Sep-Oct;4(5):343-53. doi: 10.1016/0891-0618(91)90042-b.
Receptor autoradiography allows visualization of receptor binding sites at the regional or light microscopic level. Receptor autoradiography is a mature methodology, in widespread use. It is also a dynamic and expanding methodology, benefiting constantly from the introduction of new techniques and instrumentation. In particular, receptor autoradiography has taken advantage of image analysis instrumentation to provide efficient spatial mapping of receptor populations and their pharmacological characteristics. A major contribution to the understanding of receptors has come from the recent cloning of the genes coding for many of these receptors. This has allowed the use of in situ hybridization to demonstrate the cells expressing mRNA coding for specific receptor subtypes. The result is that many receptor populations, previously thought to be homogeneous, are shown to be composed of several subtypes. As a consequence, the distribution of many receptors requires re-examination, which is aided by the development of new and more selective ligands. With the incorporation of techniques from molecular biology into receptor autoradiography, the demands upon image analysis instruments have expanded. Over the past decade, densitometric image analysers have attained a high level of sophistication for classical receptor autoradiography. However, to serve the needs of today's receptor laboratory, an image analyser must be equally capable in regional densitometry, in counting and spatial mapping of grain and/or cell locations at the microscopic level, and in analysing electrophoresis gels. Advances in image analysis hardware and software are keeping pace with the requirements of receptor laboratories.
受体放射自显影术能够在区域或光学显微镜水平上显示受体结合位点。受体放射自显影术是一种成熟的方法，被广泛应用。它也是一种动态且不断发展的方法，不断受益于新技术和仪器的引入。特别是，受体放射自显影术利用图像分析仪器来高效地对受体群体及其药理学特性进行空间定位。对受体理解的一个主要贡献来自于最近对许多这些受体编码基因的克隆。这使得能够使用原位杂交来证明表达特定受体亚型编码mRNA的细胞。结果是，许多以前被认为是同质的受体群体被证明是由几种亚型组成的。因此，许多受体的分布需要重新审视，这得益于新型且更具选择性的配体的开发。随着分子生物学技术融入受体放射自显影术，对图像分析仪器的需求也增加了。在过去十年中，密度图像分析仪在经典受体放射自显影术中已达到了很高的复杂程度。然而，为了满足当今受体实验室的需求，图像分析仪必须同样能够进行区域密度测定、在显微镜水平上对颗粒和/或细胞位置进行计数和空间定位，以及分析电泳凝胶。图像分析硬件和软件的进步与受体实验室的要求保持同步。
