Gall C, Sumikawa K, Lynch G
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Eur J Pharmacol. 1990 Sep 18;189(2-3):217-21. doi: 10.1016/0922-4106(90)90025-s.
A cDNA clone which encodes a putative kainate receptor was prepared with the polymerase chain reaction; from this, 'antisense' RNA fragments were produced and used to evaluate the distribution of mRNA for the kainate-receptor in rat brain with in situ hybridization techniques. The data demonstrate marked regional differences in the abundance of kainate receptor mRNA with highest levels present in the granule and pyramidal cell layers of hippocampus, the Purkinje cell layer of the cerebellum, and all cell layers of the olfactory bulb.
利用聚合酶链反应制备了一个编码假定的红藻氨酸受体的cDNA克隆；由此产生了“反义”RNA片段，并用于通过原位杂交技术评估大鼠脑中红藻氨酸受体mRNA的分布。数据表明，红藻氨酸受体mRNA丰度存在明显的区域差异，在海马的颗粒细胞层和锥体细胞层、小脑的浦肯野细胞层以及嗅球的所有细胞层中含量最高。
