Hong Liang-Li, Tian Dong-Ping, Su Min, Shen Xiu-Na, Gao Yuxia
Department of Pathology, Shantou University Medical College, Shantou 515031, China.
Wei Sheng Yan Jiu. 2006 Jan;35(1):54-8.
To establish the selenium (Se) deficient animal model on F344 inbred line rats and observe the effects of a long-term Se-deficiency on the offspring's neuro-behavior, abilities of learning and memory.
Feeding F344 inbred line rats on Se-deficient diet to establish Se-deficient animal model. For the offspring, the body weight, physiological indexes nervous reflections for growth and development were monitored during the early postnatal period.
The Se-deficient diet contained less than 0.01 mg/kg and the glutathione peroxidase (GSH-Px) activity in blood of the Se-deficient group rats is lower than the Se-normal group after feeding on Se-deficient diet for 4 weeks. For the offspring, the birth weight and the body weight of Se-deficient group were obviously lower than the Se-normal group before weaning. Se-deficient offspring rats differed from Se-normal controls in lower scores in surface righting reflex (RR) test at postnatal 4th day after delivery, cliff avoidance test at postnatal 7th day and auditory acuity trial at postnatal 10th day respectively. But these differences disappear after a few days in the same tests. In addition, no significant differences between two groups in suspending test and walking ability test at postnatal 12th and 14th day. In open field test, Se-deficient male offspring stayed less time in the middle grid and moved less. In Morris water maze test, the Se-deficient offspring spent more time to find the hidden platform at the 6th and 9th training tests in the place navigation trial. Furthermore, the Se-deficient group spent less time in target quadrant when giving the spatial probe trial.
A Se-deficient animal model have been established on F344 inbred line rats successfully. A long-term Se deficiency could retard the development of the offspring in uterus and after delivery. Se deficiency also decreased the offspring's abilities of spatial learning and memory in Morris water maze test and resulted in the male offspring's nervousness to new stimulant.
建立F344近交系大鼠硒缺乏动物模型，观察长期缺硒对仔代神经行为、学习记忆能力的影响。
用缺硒饲料喂养F344近交系大鼠建立缺硒动物模型。对仔代，在出生后早期监测其体重、生长发育的生理指标及神经反射。
缺硒饲料含硒量低于0.01mg/kg，缺硒组大鼠喂饲缺硒饲料4周后，其血中谷胱甘肽过氧化物酶（GSH-Px）活性低于硒正常组。对仔代，缺硒组出生体重及断奶前体重明显低于硒正常组。缺硒仔代大鼠在出生后第4天的表面翻正反射（RR）试验、第7天的避崖试验及第10天的听觉敏锐度试验中得分分别低于硒正常对照组，但在相同试验中几天后这些差异消失。此外，两组在出生后第12天和第14天的悬吊试验和行走能力试验中无显著差异。在旷场试验中，缺硒雄性仔代在中间格停留时间短且活动少。在Morris水迷宫试验中，缺硒仔代在地点导航试验的第6次和第9次训练试验中寻找隐藏平台花费时间更多。此外，在空间探索试验中，缺硒组在目标象限停留时间短。
成功建立了F344近交系大鼠缺硒动物模型。长期缺硒可延缓仔代在子宫内及出生后的发育。缺硒还降低了仔代在Morris水迷宫试验中的空间学习记忆能力，并导致雄性仔代对新刺激的紧张。
