Han Xiaojie, Hu Xiaoxia, Wei Qing, Chen Yilin, Yu De'e, Hu Qiansheng
Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Wei Sheng Yan Jiu. 2013 Nov;42(6):978-81, 986.
To study the effect of lead and (or) selenium on learning and memory ability in rats.
SPF Wistar rats, after weaning, were divided into six groups, control group, Pb group (respectively Pb exposed), Se group (respectively Se added), Pb-Se group (added Se after Pb exposure), Se-Pb group (added Se before Pb exposure) and Pb + Se group (Pb and Se exposed simultaneously). After intervention for six weeks in rats, the spatial learning and memory of each group rats were measured by Morris water maze assay.
Rats in Pb group had significantly longer latency, less site crossings, less percentage of time and distance spent in the target quadrant, and bigger first bearing compared with control group (P < 0.05). Rats in Pb and Se joint exposure groups had significantly shorter latency, more site crossings, less percentage of time and distance spent in the target quadrant, and smaller first bearing compared with Pb group (P < 0.05). There were no significant differences in the indexes of spatial learning and memory ability between the groups of lead and selenium joint exposure groups (P > 0.05).
Lead damaged the ability of learning and memory in rats and organic selenium had protective effects on Pb-induced spatial learning and memory deficits in rats.
研究铅和（或）硒对大鼠学习记忆能力的影响。
将断乳后的SPF级Wistar大鼠分为6组，即对照组、铅组（单纯染铅）、硒组（单纯加硒）、铅 - 硒组（先染铅后加硒）、硒 - 铅组（先加硒后染铅）和铅 + 硒组（铅和硒同时染毒）。大鼠干预6周后，采用Morris水迷宫实验检测各组大鼠的空间学习记忆能力。
铅组大鼠潜伏期显著延长，穿格次数减少，在目标象限停留时间和距离百分比降低，首项夹角增大，与对照组比较差异有统计学意义（P < 0.05）。铅与硒联合染毒组大鼠潜伏期显著缩短，穿格次数增多，在目标象限停留时间和距离百分比降低，首项夹角减小，与铅组比较差异有统计学意义（P < 0.05）。铅与硒联合染毒组之间空间学习记忆能力指标差异无统计学意义（P > 0.05）。
铅损害大鼠学习记忆能力，有机硒对铅所致大鼠空间学习记忆障碍具有保护作用。
