通过16S rDNA限制性片段长度多态性分析鉴定库蚊气单胞菌

Identification of Aeromonas culicicola by 16S rDNA RFLP.

作者信息

Kaznowski Adam, Konecka Edyta

机构信息

Department of Microbiology, Institute of Experimental Biology, A Mickiewicz University, Poznań, Poland.

出版信息

Pol J Microbiol. 2005;54(4):335-8.

PMID:16599307

Abstract

Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed discriminating A. culicicola from all other Aeromonas species with the exception of A. jandaei. Additional digestion of 16S rDNA PCR product with BceAI showed a possibility of distinguishing A. jandaei from A. culicicola.

摘要

开展了关于改进库蚊气单胞菌鉴定的研究。该物种在表型上与维罗纳气单胞菌嗜酒生物型、嗜酒气单胞菌和嗜异糖气单胞菌非常相似。库蚊气单胞菌分离株的16S rDNA序列与詹氏气单胞菌、维罗纳气单胞菌和豚鼠气单胞菌显示出最高的相似性。用AluI和MboI限制性内切酶消化16S rDNA PCR产物能够将库蚊气单胞菌与除詹氏气单胞菌外的所有其他气单胞菌属物种区分开来。用BceAI对16S rDNA PCR产物进行进一步消化显示出区分詹氏气单胞菌和库蚊气单胞菌的可能性。