Hasegawa Hiroshi, Takano Hiroyuki, Ohtsuka Masashi, Ueda Kazutaka, Niitsuma Yuriko, Qin Yingjie, Tadokoro Hiroyuki, Shiomi Masashi, Komuro Issei
Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
Biochem Biophys Res Commun. 2006 May 26;344(1):370-6. doi: 10.1016/j.bbrc.2006.03.081. Epub 2006 Mar 24.
Granulocyte colony-stimulating factor (G-CSF) prevents left ventricular remodeling after myocardial infarction, but its effect on atherosclerosis is unknown. We examined two kinds of rabbit atherosclerosis models. Myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHL-MI) rabbits were treated with G-CSF or saline for 7 days from 14 months old. The vascular injury models were created by inflating angioplasty balloon in the iliac artery of rabbits and were divided into G-CSF and saline group. G-CSF significantly reduced the stenosis score of coronary artery and lipid plaque area of thoracic aorta in WHHL-MI rabbits at 4 weeks after the treatment. In the vascular injury model, G-CSF significantly prevented an increase in neointima/media ratio at 4 weeks after the treatment. G-CSF accelerated the reendothelialization of denuded arteries, and the pretreatment with nitric oxide synthase inhibitor significantly inhibited it. These results suggest that G-CSF has a therapeutic potential for the progression of atherosclerosis.
粒细胞集落刺激因子（G-CSF）可预防心肌梗死后的左心室重构，但其对动脉粥样硬化的影响尚不清楚。我们研究了两种兔动脉粥样硬化模型。将易发生心肌梗死的渡边遗传性高脂血症（WHHL-MI）兔从14月龄起用G-CSF或生理盐水治疗7天。通过在兔髂动脉中充盈血管成形术球囊建立血管损伤模型，并分为G-CSF组和生理盐水组。治疗4周后，G-CSF显著降低了WHHL-MI兔冠状动脉狭窄评分和胸主动脉脂质斑块面积。在血管损伤模型中，治疗4周后，G-CSF显著阻止了内膜/中膜比值的增加。G-CSF加速了剥脱动脉的再内皮化，一氧化氮合酶抑制剂预处理可显著抑制这一过程。这些结果表明，G-CSF对动脉粥样硬化的进展具有治疗潜力。
