Kologrivova Irina, Suslova Tatiana, Koshelskaya Olga, Trubacheva Oksana, Haritonova Olga, Vinnitskaya Irina
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia.
Biomedicine (Taipei). 2020 Jun 5;10(2):36-47. doi: 10.37796/2211-8039.1015. eCollection 2020.
Monocytes are recognized as central cells in the progression of atherosclerosis, and are subcategorized into classical (CD14++CD16), intermediate (CD14++CD16) and non-classical (CD14+CD16) subsets.
The present study aimed to assess the relationships between different subsets of monocytes, metabolic and inflammatory factors in patients with stable coronary heart disease.
A total of 26 patients (both men and women) with stable ischemic heart disease (IHD) were recruited. Among all the recruited patients, 17 patients had significant coronary artery disease defined as diameter stenosis more than 70%. Severity of CHD was assessed by the Gensini score (GS). Counts of CD14++CD16, CD14++CD16, and CD14+CD16 monocytes were evaluated by flow cytometry. Gating was verified and expression of CD163 was determined by imaging flow cytometry. Key cardiac markers, cytokines, and chemokines were detected in serum and in 24-hour-culture medium for peripheral blood mononuclear cells (PBMC) by multiplex analysis. The Mann-Whitney U-test and Spearman's rank correlation coefficient (r) were used for statistical analysis.
Patients with stenosis <70% tended to have higher frequency of CD14+CD16 monocytes compared to patients with coronary artery stenosis >70%. The frequencies of CD163+CD14++CD16 and CD163+CD14+CD16 monocytes were elevated in patients with stenosis >70%. In patients with stenosis <70%, the frequency of classical monocytes positively correlated and the frequency of non-classical monocytes negatively correlated with the value of GS (R =0.757; p =0.018 and R = -0.757; p = 0.018, respectively).
In patients with ischemic heart disease, the frequency of classical monocytes was directly correlated with the severity of atherosclerosis, while the frequency of non-classical monocytes was correlated inversely. The effects of these monocyte subsets in the development of myocardial ischemia still need to be elucidated.
单核细胞被认为是动脉粥样硬化进展中的核心细胞，并被细分为经典型（CD14++CD16）、中间型（CD14++CD16）和非经典型（CD14+CD16）亚群。
本研究旨在评估稳定型冠心病患者不同单核细胞亚群与代谢及炎症因子之间的关系。
共招募了26例稳定型缺血性心脏病（IHD）患者（男女均有）。在所有招募的患者中，17例患者患有严重冠状动脉疾病，定义为直径狭窄超过70%。冠心病的严重程度通过Gensini评分（GS）进行评估。通过流式细胞术评估CD14++CD16、CD14++CD16和CD14+CD16单核细胞的计数。通过成像流式细胞术验证门控并确定CD163的表达。通过多重分析检测血清和外周血单个核细胞（PBMC）24小时培养基中的关键心脏标志物、细胞因子和趋化因子。采用Mann-Whitney U检验和Spearman等级相关系数（r）进行统计分析。
与冠状动脉狭窄>70%的患者相比，狭窄<70%的患者CD14+CD16单核细胞频率往往更高。狭窄>70%的患者中，CD163+CD14++CD16和CD163+CD14+CD16单核细胞频率升高。在狭窄<??70%的患者中，经典单核细胞频率与GS值呈正相关，非经典单核细胞频率与GS值呈负相关（R =0.757；p =0.018和R = -0.757；p = 0.018）。
在缺血性心脏病患者中，经典单核细胞频率与动脉粥样硬化严重程度直接相关，而非经典单核细胞频率呈负相关。这些单核细胞亚群在心肌缺血发展中的作用仍需阐明。
