Weiss David J, Austria Eric J, Anderton Christopher R, Hompesch Richard, Jander Ashley
Department of Chemistry, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
J Chromatogr A. 2006 Jun 2;1117(1):103-8. doi: 10.1016/j.chroma.2006.03.057. Epub 2006 Apr 4.
Dietary supplements are growing in popularity as a source of catechins such as epigallocatechin gallate (EGCG). The first determination of five catechins in green tea extract dietary supplements using an extraction followed by micellar electrokinetic chromatography (MEKC) with UV detection is presented here. The optimum run buffer is 5 mM borate-60 mM phosphate with 50 mM SDS at pH 7.00 with detection at 210 nm. The limit of detection is 2-3 microg/mL (S/N=3) and the limit of quantitation is 6-8 microg/mL (S/N = 10). Results indicate that the amount of catechins varies greatly among manufacturers, between capsules of the same manufacturers, and between batches.
作为表没食子儿茶素没食子酸酯（EGCG）等儿茶素的来源，膳食补充剂越来越受欢迎。本文首次报道了采用萃取后结合紫外检测的胶束电动色谱法（MEKC）测定绿茶提取物膳食补充剂中五种儿茶素的方法。最佳运行缓冲液为5 mM硼酸盐 - 60 mM磷酸盐，含50 mM十二烷基硫酸钠（SDS），pH值为7.00，检测波长为210 nm。检测限为2 - 3 μg/mL（信噪比 = 3），定量限为6 - 8 μg/mL（信噪比 = 10）。结果表明，不同制造商之间、同一制造商的不同胶囊之间以及不同批次之间，儿茶素的含量差异很大。
