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胶束电动色谱法测定抹茶绿茶中的儿茶素

Determination of catechins in matcha green tea by micellar electrokinetic chromatography.

作者信息

Weiss David J, Anderton Christopher R

机构信息

Department of Chemistry, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.

出版信息

J Chromatogr A. 2003 Sep 5;1011(1-2):173-80. doi: 10.1016/s0021-9673(03)01133-6.

DOI:10.1016/s0021-9673(03)01133-6
PMID:14518774
Abstract

Catechins in green tea are known to have many beneficial health properties. Recently, it has been suggested that matcha has greater potential health benefits than other green teas. Matcha is a special powdered green tea used in the Japanese tea ceremony. However, there has been no investigation to quantitate the catechin intake from matcha compared to common green teas. We have developed a rapid method of analysis of five catechins and caffeine in matcha using micellar electrokinetic chromatography. Results are presented for water and methanol extractions of matcha compared with water extraction of a popular green tea. Using a mg catechin/g of dry leaf comparison, results indicate that the concentration of epigallocatechin gallate (EGCG) available from drinking matcha is 137 times greater than the amount of EGCG available from China Green Tips green tea, and at least three times higher than the largest literature value for other green teas.

摘要

众所周知,绿茶中的儿茶素具有许多有益健康的特性。最近,有人提出抹茶比其他绿茶具有更大的潜在健康益处。抹茶是一种用于日本茶道的特殊粉末状绿茶。然而,与普通绿茶相比,尚未有对抹茶中儿茶素摄入量进行定量的研究。我们开发了一种使用胶束电动色谱法快速分析抹茶中五种儿茶素和咖啡因的方法。给出了抹茶水提物和甲醇提取物与一种流行绿茶水提取物的比较结果。以每克干叶中儿茶素毫克数进行比较,结果表明,饮用抹茶可获得的表没食子儿茶素没食子酸酯(EGCG)浓度比中国绿尖绿茶中可获得的EGCG量高137倍,且至少比其他绿茶的最大文献值高三倍。

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