Xi Lingling, Wu Genfu, Zhu Yan
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
J Chromatogr A. 2006 May 19;1115(1-2):202-7. doi: 10.1016/j.chroma.2006.02.093. Epub 2006 Apr 4.
A new method for determination of etimicin's (ETM) purity and content is developed by liquid chromatography (LC) and pulsed amperometric detection (PAD). A reversed-phase ion-pair LC method with pulsed amperometric detection on a gold electrode after post-added NaOH is described. The mobile phase consisted of an aqueous solution containing 0.033 mol L(-1) oxalic acid, 0.012 mol L(-1) heptafluorobutyric acid, and 210 mL L(-1) acetonitrile with pH 3.40 adjusting by dilute NaOH solution. The total analysis time was not more than 30 min. The effects of the different chromatographic parameters on the separation were also investigated. A number of commercial samples of etimicin sulfate were analyzed using this method.
建立了一种采用液相色谱（LC）和脉冲安培检测（PAD）测定依替米星（ETM）纯度和含量的新方法。描述了一种在加入NaOH后采用金电极上的脉冲安培检测的反相离子对LC方法。流动相由含有0.033 mol L⁻¹草酸、0.012 mol L⁻¹七氟丁酸和210 mL L⁻¹乙腈的水溶液组成，用稀NaOH溶液调节pH至3.40。总分析时间不超过30分钟。还研究了不同色谱参数对分离的影响。使用该方法分析了多个硫酸依替米星商业样品。
