Kwon Ha-Jeong, Jeong Ji-Seon, Lee Yong-Moon, Hong Seon-Pyo
Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
J Chromatogr A. 2008 Mar 28;1185(2):251-7. doi: 10.1016/j.chroma.2008.01.052. Epub 2008 Jan 31.
We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively.
我们开发了一种反相高效液相色谱-脉冲安培检测法（RP-HPLC-PAD）用于测定糖苷。该方法灵敏、可重复且具有选择性，无需预处理步骤。使用水-乙腈梯度作为洗脱剂，人参皂苷在50分钟内完全分离，并在NaOH碱性条件下通过PAD进行检测。人参皂苷的检测限（S/N=3）为0.02 - 0.07 ng，定量限（S/N=10）为0.1 - 0.2 ng。浓度在1至50μg/mL之间时，线性回归系数为0.9984 - 0.9998。人参根和十全大补汤提取物的日内和日间精密度（RSD）均小于6.35%。人参根和十全大补汤提取物的平均回收率分别为98.19 - 105.45%和96.89 - 102.22%。
