Cajaraville Miren P, Ortiz-Zarragoitia Maren
Biologia Zelularra eta Histologia Laborategia, Zoologia eta Biologia Zelularra Saila, Zientzia eta Teknologia Fakultatea, Euskal Herriko Unibertsitatea, UPV/EHU, 644 P.K., E-48080 Bilbao, Basque Country, Spain.
Aquat Toxicol. 2006 Jun 1;78 Suppl 1:S117-23. doi: 10.1016/j.aquatox.2006.02.016. Epub 2006 Apr 5.
Peroxisome proliferation has been proposed as novel biomarker of exposure to organic pollutants in aquatic organisms. Peroxisome proliferator compounds comprise a heterogeneous group of substances known for their ability to cause massive proliferation of peroxisomes and liver carcinogenesis in sensitive species such as rodents. Recently, several marine organisms (mussels and fish) have been shown as target species of peroxisome proliferators. In the present work, we aimed to investigate the specificity of the peroxisome proliferation response in mussels. For this purpose, mussels (Mytilus edulis) were exposed for three weeks to North Sea crude oil (NSO), a mixture of NSO, alkylphenols and extra PAHs (MIX), diallylphthalate (DAP), bisphenol-A (BPA) and tetrabromodiphenylether (TBDE), or transplanted for three weeks to four stations showing different copper concentrations in a copper mine. Peroxisome proliferation was assessed by measuring the activity of the peroxisomal beta-oxidation enzyme acyl-CoA oxidase (AOX) and the volume density occupied by peroxisomes (V(VP)) in the digestive gland. Mussels exposed to NSO and MIX showed significantly increased AOX activities and V(VP) compared to control animals. Significantly higher V(VP) was also found in DAP and TBDE exposed mussels. V(VP) did not vary in mussels transplanted into a copper concentration gradient. Our results confirm the usefulness and specificity of peroxisome proliferation as a suitable biomarker of exposure to organic contaminants such as oil derived hydrocarbons, phthalate plasticizers and polybrominated flame retardants in mussels.
过氧化物酶体增殖已被提议作为水生生物接触有机污染物的新型生物标志物。过氧化物酶体增殖剂化合物是一类异质性物质，以其能在啮齿动物等敏感物种中引起过氧化物酶体大量增殖和肝脏致癌作用而闻名。最近，几种海洋生物（贻贝和鱼类）已被证明是过氧化物酶体增殖剂的靶标物种。在本研究中，我们旨在研究贻贝过氧化物酶体增殖反应的特异性。为此，将贻贝（紫贻贝）暴露于北海原油（NSO）、NSO、烷基酚和额外多环芳烃的混合物（MIX）、邻苯二甲酸二烯丙酯（DAP）、双酚A（BPA）和四溴二苯醚（TBDE）三周，或移植到一个铜矿中四个铜浓度不同的站点三周。通过测量消化腺中过氧化物酶体β-氧化酶酰基辅酶A氧化酶（AOX）的活性和过氧化物酶体所占的体积密度（V(VP)）来评估过氧化物酶体增殖。与对照动物相比，暴露于NSO和MIX的贻贝显示AOX活性和V(VP)显著增加。在暴露于DAP和TBDE的贻贝中也发现V(VP)显著更高。移植到铜浓度梯度中的贻贝的V(VP)没有变化。我们的结果证实了过氧化物酶体增殖作为贻贝接触有机污染物（如石油衍生烃、邻苯二甲酸酯增塑剂和多溴化阻燃剂）的合适生物标志物的有用性和特异性。
