Badhan A K, Chadha B S, Kaur Jatinder, Saini H S, Bhat M K
Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.
Bioresour Technol. 2007 Feb;98(3):504-10. doi: 10.1016/j.biortech.2006.02.009. Epub 2006 Apr 4.
This study reports the production of xylanolytic and cellulolytic enzymes by a thermophilic fungal isolate Myceliophthora sp. using a cheap medium containing rice straw and chemically defined basal medium under solid-state culture. A combination of one factor at a time approach followed by response surface methodology using Box-Behnken design of experiments resulted in 2.5, 1.25, 1.28 and 4.23 fold increase in xylanase, endoglucanase, beta-glucosidase and FPase activity, respectively. The zymograms developed against IEF gels showed that multiple isoforms of xylanase (5), endoglucanase (4) and beta-glucosidase (2) were produced under optimized culture conditions. Moreover, thiol containing serine proteases produced during the growth of the culture had no role in the post-translational modification of these xylanases.
本研究报道了嗜热真菌菌株嗜热毁丝霉（Myceliophthora sp.）在固态培养条件下，利用含有稻草的廉价培养基和化学成分明确的基础培养基生产木聚糖酶和纤维素酶的情况。采用一次一个因素法，随后使用Box-Behnken实验设计的响应面方法，结果木聚糖酶、内切葡聚糖酶、β-葡萄糖苷酶和滤纸酶活性分别提高了2.5倍、1.25倍、1.28倍和4.23倍。针对IEF凝胶制作的酶谱显示，在优化培养条件下产生了多种木聚糖酶同工型（5种）、内切葡聚糖酶同工型（4种）和β-葡萄糖苷酶同工型（2种）。此外，培养过程中产生的含硫醇丝氨酸蛋白酶在这些木聚糖酶的翻译后修饰中不起作用。
