Li Aiqun, May Matthew P, Bigelow James C
Department of Pharmaceutical Sciences, College of Pharmacy, Idaho State University, Pocatello, ID 83209-8334, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 19;836(1-2):129-32. doi: 10.1016/j.jchromb.2006.03.041. Epub 2006 Apr 4.
Atrazine is an herbicide which has shown potential antimalarial effects both in vitro and in vivo in rats. In order to study the metabolism of atrazine in rat livers, we developed a sensitive LC/MS/MS method for the identification of atrazine and several of its metabolites. Using this method, we identified one previously unreported metabolite with a mass of 211 Da in addition to two known metabolites. This new metabolite was confirmed to be N-ethyl-6-methoxy-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine, also known as atraton, by comparison of the LC/MS/MS mass spectra and the retention time to those of a commercial standard.
阿特拉津是一种除草剂，已在体外和大鼠体内显示出潜在的抗疟作用。为了研究阿特拉津在大鼠肝脏中的代谢，我们开发了一种灵敏的液相色谱/串联质谱法来鉴定阿特拉津及其几种代谢产物。使用该方法，除了两种已知代谢产物外，我们还鉴定出一种质量为211道尔顿的先前未报道的代谢产物。通过将液相色谱/串联质谱的质谱图和保留时间与商业标准品的进行比较，确认这种新的代谢产物为N-乙基-6-甲氧基-N'-(1-甲基乙基)-1,3,5-三嗪-2,4-二胺，也称为莠去通。
