Bi Anding, Cui Jinjuan, Ma Yu-Ping, Olshevskaya Elena, Pu Mingliang, Dizhoor Alexander M, Pan Zhuo-Hua
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Neuron. 2006 Apr 6;50(1):23-33. doi: 10.1016/j.neuron.2006.02.026.
The death of photoreceptor cells caused by retinal degenerative diseases often results in a complete loss of retinal responses to light. We explore the feasibility of converting inner retinal neurons to photosensitive cells as a possible strategy for imparting light sensitivity to retinas lacking rods and cones. Using delivery by an adeno-associated viral vector, here, we show that long-term expression of a microbial-type rhodopsin, channelrhodopsin-2 (ChR2), can be achieved in rodent inner retinal neurons in vivo. Furthermore, we demonstrate that expression of ChR2 in surviving inner retinal neurons of a mouse with photoreceptor degeneration can restore the ability of the retina to encode light signals and transmit the light signals to the visual cortex. Thus, expression of microbial-type channelrhodopsins, such as ChR2, in surviving inner retinal neurons is a potential strategy for the restoration of vision after rod and cone degeneration.
视网膜退行性疾病导致的光感受器细胞死亡常常会致使视网膜对光的反应完全丧失。我们探索将视网膜内层神经元转化为光敏感细胞的可行性，以此作为一种可能的策略，为缺乏视杆细胞和视锥细胞的视网膜赋予光敏感性。通过腺相关病毒载体进行递送，在此我们表明，微生物型视紫红质——通道视紫红质-2（ChR2）——能够在啮齿动物的视网膜内层神经元中实现长期表达。此外，我们证明，在患有光感受器退化的小鼠存活的视网膜内层神经元中表达ChR2，可以恢复视网膜编码光信号并将光信号传递至视觉皮层的能力。因此，在存活的视网膜内层神经元中表达诸如ChR2等微生物型通道视紫红质，是视杆细胞和视锥细胞退化后恢复视力的一种潜在策略。
