Hurbánková Marta, Cerná Silvia, Gergelová Petra, Wimmerová Sona
Research Base of the Slovak Medical University, Bratislava, Slovakia.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Dec;149(2):367-71.
Industrial fibrous dusts are applied in many industrial branches and represent adverse factors in occupational and environmental area. Refractory ceramic fibers (RCFs) - amorphous alumina silicates - are used as one kind of asbestos substitutes. Because RCFs are relatively durable and some RCFs are respirable, they may present a potential health hazard by inhalation. The aim of present work was to find out the subchronic effect of RCFs on selected parameters of bronchoalveolar lavage (BAL) in W-rats, confirm the biopersistence of RCFs after 6 month instillation and contribute to the understanding of the pathomechanism of lung injury after fibrous dust exposure. Wistar rats were intratracheally instilled with 4 mg/animal of RCFs - exposed group and with 0.4 ml saline solution/animal - control group. Animals were sacrificed after 6 month exposure. Bronchoalveolar lavage (BAL) was performed and selected BAL parameters (mainly inflammatory and cytotoxic) were examined. After treatment with RCFs the following changes were observed: statistically significant increase in proportion of lymphocytes and polymorphonuclears as well as in % of immature alveolar macrophages (AM) and phagocytic activity of AM; statistically significant decrease in viability of AM and proportion of AM (from the differential cell count) in comparison with the control group. The results of this study indicated that RCFs even 6 months after intratracheal instillation very significantly changed the majority of examined BAL parameters. The presence of inflammatory and cytotoxic response in lung may signalize beginning or developing disease process.
工业纤维性粉尘应用于许多工业部门，是职业和环境领域的不利因素。耐火陶瓷纤维（RCFs）——无定形硅酸铝——被用作一种石棉替代品。由于RCFs相对耐用，且一些RCFs可吸入，它们可能通过吸入造成潜在的健康危害。本研究的目的是了解RCFs对W大鼠支气管肺泡灌洗（BAL）选定参数的亚慢性影响，确认滴注6个月后RCFs的生物持久性，并有助于理解纤维性粉尘暴露后肺损伤的发病机制。将4mg/只的RCFs经气管内滴注到Wistar大鼠——暴露组，将0.4ml生理盐水/只滴注到对照组。暴露6个月后处死动物。进行支气管肺泡灌洗（BAL），并检查选定的BAL参数（主要是炎症和细胞毒性参数）。用RCFs处理后观察到以下变化：淋巴细胞和多形核细胞比例以及未成熟肺泡巨噬细胞（AM）百分比和AM吞噬活性有统计学意义的增加；与对照组相比，AM活力和AM比例（来自细胞分类计数）有统计学意义的降低。本研究结果表明，即使在经气管内滴注6个月后，RCFs仍非常显著地改变了大多数检测的BAL参数。肺中炎症和细胞毒性反应的存在可能表明疾病过程的开始或发展。
