Iglesias I, León D, Ruiz M A, Albasanz J L, Martín M
Department of Inorganic, Organic and Biochemistry, Faculty of Chemical Sciences, Centro Regional de Investigaciones Biomédicas, Ciudad Real, Spain.
Amino Acids. 2006 May;30(3):257-66. doi: 10.1007/s00726-005-0293-7. Epub 2006 Apr 13.
Caffeine is the most widely consumed substance in the world which antagonizes adenosine effects. Adenosine acting through A(1) receptors inhibits glutamate release which binds to metabotropic glutamate receptors (mGluRs). Recently, we have shown that maternal caffeine intake during gestation causes down-regulation of A(1) and metabotropic glutamate receptors in the brain of both rat mothers and fetuses. In the present work we provide evidence that caffeine also affects receptors in hearts, causing a decrease in mGluRs from both maternal and fetal hearts. A decrease in G(q/11) and PLC beta(1) proteins level was also observed in both tissues. However, phospholipase C activity was only affected in fetal heart, being significantly decreased. These results suggest an in vivo cross-talk mechanism between adenosine and glutamate receptors in peripheral tissues. Therefore, special attention should be paid to caffeine ingestion during gestation.
咖啡因是世界上消费最为广泛的可拮抗腺苷作用的物质。通过A(1)受体起作用的腺苷可抑制谷氨酸释放，而谷氨酸会与代谢型谷氨酸受体（mGluRs）结合。最近，我们发现孕期母体摄入咖啡因会导致大鼠母体和胎儿大脑中A(1)受体和代谢型谷氨酸受体下调。在本研究中，我们提供证据表明咖啡因也会影响心脏中的受体，导致母体和胎儿心脏中的mGluRs减少。在这两种组织中还观察到G(q/11)和PLCβ(1)蛋白水平降低。然而，磷脂酶C活性仅在胎儿心脏中受到影响，显著降低。这些结果表明外周组织中腺苷和谷氨酸受体之间存在体内相互作用机制。因此，孕期应特别注意咖啡因的摄入。
