Departamento de Química Inorgánica, Orgánica y Bioquímica, Facultad de Químicas, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Avenida Camilo José Cela, Ciudad Real, Spain.
J Neurosci Res. 2010 May 1;88(6):1252-61. doi: 10.1002/jnr.22287.
Even though caffeine can be excreted in breast milk, few studies have analyzed the effect of maternal caffeine consumption during lactation on neonatal brain. In the present work pregnant rats were treated daily with 1 g/L of caffeine in their drinking water during pregnancy and/or lactation and the effect on adenosine A(1) receptor in brains from both lactating mothers and 15 days-old neonates was assayed using radioligand binding and real time PCR assays. Mothers receiving caffeine during gestational period developed motor activation in gestational days 8-10 which was associated with a significant decrease of total adenosine A(1) receptor number (84%). A similar decrease was detected in mothers treated with caffeine during lactation (76%) and throughout gestation and lactation (73%); this was accompanied by a significant decrease in mRNA level coding adenosine A(1) receptor (28%). In male neonates, adenosine A(1) receptor was also decreased after chronic caffeine exposure during gestation (80%), lactation (76%) and gestation plus lactation (80%). In female neonates, adenosine A(1) receptor tended to decrease in response to caffeine exposure although no significant variations were found. No variation in the level of mRNA coding adenosine A(1) receptor was detected in neonates in any case. Concerning adenosine A(2A) receptor, radioligand binding assays revealed that this receptor remains unaltered in maternal and neonatal brain in response to caffeine exposure. However, caffeine consumption during gestation and lactation evoked a significant decrease in mRNA level coding A(2A) receptor (32%) in mothers' brain.
尽管咖啡因可以从母乳中排出，但很少有研究分析哺乳期母亲咖啡因摄入对新生儿大脑的影响。在本工作中，妊娠大鼠在妊娠和/或哺乳期每天饮用 1g/L 咖啡因水，并用放射性配体结合和实时 PCR 检测分析对哺乳期母亲和 15 日龄新生大鼠大脑中腺苷 A(1)受体的影响。在妊娠第 8-10 天接受咖啡因的母亲表现出运动激活，这与总腺苷 A(1)受体数量的显著减少（84%）有关。在哺乳期接受咖啡因治疗的母亲（76%）和整个妊娠期和哺乳期（73%）也检测到类似的减少；这伴随着编码腺苷 A(1)受体的 mRNA 水平的显著降低（28%）。在雄性新生儿中，慢性暴露于咖啡因也会导致腺苷 A(1)受体减少（妊娠期 80%，哺乳期 76%，妊娠期和哺乳期 80%）。在雌性新生儿中，腺苷 A(1)受体也倾向于减少，但没有发现显著变化。在任何情况下，在新生儿中都没有检测到编码腺苷 A(1)受体的 mRNA 水平的变化。关于腺苷 A(2A)受体，放射性配体结合试验显示，这种受体在母体和新生大鼠大脑中对咖啡因暴露保持不变。然而，在妊娠期和哺乳期摄入咖啡因会导致母亲大脑中编码 A(2A)受体的 mRNA 水平显著下降（32%）。
