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[对原多核变形虫(格鲁伯,1884年)(多核鞭毛虫纲,原生物目)的光学显微镜和电子显微镜研究]

[Light and electron microscopic investigation of Pelomyxa prima (Gruber, 1884) (Peloflagellatea, Pelobiontida)].

作者信息

Frolov A O, Chistiakova L V, Malysheva M N, Gudkov A V

出版信息

Tsitologiia. 2005;47(1):89-98.

PMID:16602249
Abstract

Cell organization of a multinuclear pelobiont Pelomyxa prima has been studied at the light and electron microscopic levels. Motile individuals demonstrate a characteristic drop-like or pyriform shape and reach 550 microkm in length. The cell cover is represented by a well-developed, morphologically differentiated glycocalyx 80-100 nm thick. The cytoplasm contains many structural vacuoles. The nuclei are of vertical type, numbering up to several nuclei in large individuals. Numerous cytoplasmic microtubules are associated with the external membrane of the nuclear envelope. Separate non-motile flagella are distributed throughout the cell surface, being more numerous in the posterior body end and uroidal zone of the protist. Basal bodies of the flagella are extremely long, being deeply immersed into the cytoplasm. These bodies are surrounded by a muff of electron-dense material, with numerous microtubules radiating from it. A compact bundle of microtubules starts from the base of a basal body axially into the cytoplasm. Besides, a band-like lateral microtubular rootlet is present. The number of microtubules in the axoneme of undulipodia is unstable. Neither mitochondria, nor Golgi complex were found. Two species of bacterial endocytobionts are present in the cytoplasm in considerable numbers.

摘要

多核裸变形虫Pelomyxa prima的细胞组织已在光学和电子显微镜水平上进行了研究。游动个体呈现出典型的水滴状或梨形,长度可达550微米。细胞覆盖物由一层发育良好、形态上有分化的糖萼代表,其厚度为80 - 100纳米。细胞质中含有许多结构液泡。细胞核为垂直型,在大型个体中数量可达几个。许多细胞质微管与核膜的外膜相关联。单独的非游动鞭毛分布在整个细胞表面,在原生生物的身体后端和尾状区更为密集。鞭毛的基体极长,深深嵌入细胞质中。这些基体被一团电子致密物质包围,有许多微管从这里辐射出来。一束紧密的微管从基体底部轴向延伸到细胞质中。此外,还存在一条带状的横向微管小根。波动足轴丝中的微管数量不稳定。未发现线粒体和高尔基体复合体。细胞质中存在大量两种细菌内共生体。

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