Baumstark J S, Chang R S
Department of Obstetrics and Gynecology, Creighton University, School of Medicine, Omaha, Nebraska 68131.
Appl Theor Electrophor. 1990;1(4):205-6.
A convenient and rapid method for casting of narrow-bore (1.5-2.5 mm, i.d.) first dimension isoelectric focusing gel tubes is described. The procedure utilizes a peristaltic pump and a 2-inch, 23 gauge blunt needle for dispensing the gel into fixed tubes on a 1.1 degree incline. The method is simple to perform, precludes the formation of air bubbles and little or no manual dexterity is required.
