Parekh Harendra S, Marano Robert J, Rakoczy Elizabeth P, Blanchfield Joanne, Toth Istvan
School of Pharmacy, The University of Queensland, St Lucia, Qld 4072, Australia.
Bioorg Med Chem. 2006 Jul 15;14(14):4775-80. doi: 10.1016/j.bmc.2006.03.029. Epub 2006 Apr 5.
This article follows on from our previous work in the area of non-viral gene delivery using polycationic dendrimers (PCDs). Herein we report on the synthesis and efficacy of a new library of lipid core PCDs in the delivery of the anti-angiogenic oligonucleotide (ODN-1) to retinal pigment epithelial cells. ELISA was used to monitor hVEGF levels in cells transfected with dendriplexes, Cytofectin GSV and control (non-transfected). At 48 h, hVEGF titres had returned to that of the untransfected control for Cytofectin GSV however, a number of dendriplexes continued to exhibit a marked reduction in hVEGF titres.
本文延续了我们之前在使用聚阳离子树枝状大分子（PCD）进行非病毒基因递送领域的工作。在此，我们报告了一个新的脂质核心PCD文库在将抗血管生成寡核苷酸（ODN - 1）递送至视网膜色素上皮细胞方面的合成及效果。采用酶联免疫吸附测定（ELISA）来监测用树枝状复合物、细胞转染试剂Cytofectin GSV转染的细胞以及对照（未转染）细胞中的人血管内皮生长因子（hVEGF）水平。在48小时时，对于细胞转染试剂Cytofectin GSV，hVEGF滴度已恢复到未转染对照的水平，然而，一些树枝状复合物的hVEGF滴度仍持续显著降低。
