Lee Kui Jae, Choi Min Kyung, Lee Wang Hyu, Rajkumar Mani
Division of Bioresources Science, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, South Korea.
Virus Genes. 2006 Apr;32(2):171-6. doi: 10.1007/s11262-005-6873-5.
The complete sequences of both RNAs of an isolate of barley yellow mosaic virus (BaYMV) from Haenam, Korea, were determined. RNA1 is 7639 nucleotides long [excluding the 3'-poly(A)], and codes for a 270 kDa polyprotein of 2411 amino acids which contains the capsid protein (CP) at the C terminus and seven putative non-structural proteins. RNA2 is 3582 nucleotides long and codes for a polyprotein of 890 amino acids, which contains a 28 kDa putative proteinase (P1) and a 73 kDa polypeptide (P2). The whole sequences of Korean isolate (BaYMV-K) closely resembled those of an isolate from Japan (BaYMV-J) (99.6 identical nucleotides for RNA1; 99.4 for RNA2) and china (BaYMV-C) (96.7 and 96.2%, respectively) than from Germany (BaYMV-G) (93.6 and 90.4%, respectively). The greatest differences between the BaYMV-K and BaYMV-J isolates were in the 3'-NCRs of RNA1 and 5' NCRs of RNA2 and there were also some other regions of difference in Nib Pro (RNA1) and P1 (RNA2). Further, the phylogenetic analysis of CP region showed that Asian and European isolates formed distinct clusters. However, molecular variations between isolates could not be linked to earlier results showing differences in cultivar response.
测定了来自韩国海南的一株大麦黄花叶病毒（BaYMV）两个RNA的完整序列。RNA1长7639个核苷酸[不包括3'-聚腺苷酸（polyA）]，编码一个由2411个氨基酸组成的270 kDa多聚蛋白，该多聚蛋白在C端含有衣壳蛋白（CP）和7个推定的非结构蛋白。RNA2长3582个核苷酸，编码一个由890个氨基酸组成的多聚蛋白，其中含有一个28 kDa的推定蛋白酶（P1）和一个73 kDa的多肽（P2）。韩国分离株（BaYMV-K）的全序列与来自日本的分离株（BaYMV-J）（RNA1有99.6%的相同核苷酸；RNA2有99.4%）和中国的分离株（BaYMV-C）（分别为96.7%和96.2%）的序列比与来自德国的分离株（BaYMV-G）（分别为93.6%和90.4%）的序列更相似。BaYMV-K和BaYMV-J分离株之间最大的差异在于RNA1的3'-非编码区（NCR）和RNA2的5'-NCR，并且在Nib Pro（RNA1）和P1（RNA2）中也有其他一些差异区域。此外，CP区域的系统发育分析表明亚洲和欧洲分离株形成了不同的簇。然而，分离株之间的分子变异与早期显示品种反应差异的结果没有关联。
