Mitchell S B, Sanders J E
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
J Biomed Mater Res A. 2006 Jul;78(1):110-20. doi: 10.1002/jbm.a.30673.
The purpose of this research was to develop a system for controlled electrospinning of fibro-porous scaffolds for tissue engineering applications and to use this system to assess mesh architecture sensitivity to manufacturing parameters. The intent was to achieve scaffolds with well-controlled fiber diameters and inter-fiber spacing. To accomplish these objectives, a custom, closed-loop controlled, electrospinning system was built. The system was unique in that it had a collection surface that was independent of the electrodes. The system allowed independent manipulation and analysis of a number of manufacturing parameters: distance between the electrodes, distance from the nozzle to the collection surface, applied voltage, temperature of the melt, collection surface dielectric strength, and collection surface area. Morphological analysis of fabricated meshes showed that all test parameters significantly affected fiber diameter and inter-fiber spacing. Further, contrary to what is generally accepted in the electrospinning literature, voltage and temperature (inversely related to viscosity) were not the most significant parameters. Features of the collection surface, including dielectric strength and surface area, were more significant. This dominance is, in part, a reflection of the unique electrospinning system used. The collection surface, which was not connected to either of the electrodes, substantially altered the electric field between the electrodes. Using the developed controlled electrospinning system, thermoplastic polyurethane meshes with fiber diameters ranging from 5 to 18 microm with variability less than 1.8% were made; inter-fiber spacing ranged from 4 to 90 microm with variability less than 20.2%. The system has potential use in biomedical applications where meshes with controlled fiber diameter and inter-fiber spacing are of interest.
本研究的目的是开发一种用于组织工程应用的纤维多孔支架的可控静电纺丝系统，并使用该系统评估网格结构对制造参数的敏感性。目的是获得具有良好控制的纤维直径和纤维间距的支架。为实现这些目标，构建了一个定制的、闭环控制的静电纺丝系统。该系统的独特之处在于其收集表面独立于电极。该系统允许对多个制造参数进行独立操作和分析：电极之间的距离、喷嘴到收集表面的距离、施加电压、熔体温度、收集表面介电强度和收集表面积。对制成的网格进行形态分析表明，所有测试参数均对纤维直径和纤维间距有显著影响。此外，与静电纺丝文献中普遍接受的情况相反，电压和温度（与粘度成反比）并非最显著的参数。收集表面的特性，包括介电强度和表面积，更为显著。这种主导地位部分反映了所使用的独特静电纺丝系统。未与任何一个电极相连的收集表面极大地改变了电极之间的电场。使用所开发的可控静电纺丝系统，制备出了纤维直径在5至18微米之间、变异性小于1.8%的热塑性聚氨酯网格；纤维间距在4至90微米之间、变异性小于20.2%。该系统在对具有可控纤维直径和纤维间距的网格感兴趣的生物医学应用中具有潜在用途。
