Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
Biomed Mater. 2010 Oct;5(5):054110. doi: 10.1088/1748-6041/5/5/054110. Epub 2010 Sep 28.
Electrospinning of fibrous tissue engineering scaffolds has been traditionally conducted using positive voltages. In the current study, positive voltage (PV) electrospinning and negative voltage (NV) electrospinning were investigated for forming fibrous membranes of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV). In both PV-electrospinning and NV-electrospinning, the fiber diameter generally increased with increasing needle inner diameter and PHBV concentration but decreased with increasing working distance. The use of a conductivity-enhancing surfactant, benzyl triethylammonium chloride (BTEAC), significantly reduced PHBV fiber diameters from the micron scale to the sub-micron scale. Interestingly, with increasing applied voltage, the fiber diameter increased for PV-electrospinning but decreased for NV-electrospinning. The PV-electrospun fibrous membranes from solutions without BTEAC (PVEfm) and with BTEAC (PVEfm-B) and NV-electrospun membranes from solutions without BTEAC (NVEfm) and with BTEAC (NVEfm-B) were characterized in terms of their structure, wettability, thermal properties and tensile properties. Both PVEfm and NVEfm exhibited similar water contact angles (∼104°) but the contact angle of PVEfm-B or NVEfm-B was not measurable. The elongation at break of PVEfm-B or NVEfm-B was significantly higher than that of PVEfm or NVEfm. Using NV-electrospinning or a combination of NV- and PV-electrospinning may be very useful for developing suitable scaffolds for tissue engineering applications.
传统上，采用正电压进行纤维组织工程支架的静电纺丝。在本研究中，研究了正电压（PV）静电纺丝和负电压（NV）静电纺丝在形成聚（羟基丁酸-co-羟基戊酸）（PHBV）纤维膜中的应用。在 PV 静电纺丝和 NV 静电纺丝中，纤维直径通常随针内径和 PHBV 浓度的增加而增大，随工作距离的增加而减小。使用导电性增强的表面活性剂苄基三乙基氯化铵（BTEAC），可将 PHBV 纤维直径从微米级降至亚微米级。有趣的是，随着施加电压的增加，PV 静电纺丝的纤维直径增加，而 NV 静电纺丝的纤维直径减小。未添加 BTEAC 的 PV 静电纺丝纤维膜（PVEfm）和添加 BTEAC 的 PV 静电纺丝纤维膜（PVEfm-B）以及未添加 BTEAC 的 NV 静电纺丝纤维膜（NVEfm）和添加 BTEAC 的 NV 静电纺丝纤维膜（NVEfm-B）的结构、润湿性、热性能和拉伸性能进行了表征。PVEfm 和 NVEfm 的水接触角相似（约 104°），但 PVEfm-B 或 NVEfm-B 的接触角无法测量。PVEfm-B 或 NVEfm-B 的断裂伸长率明显高于 PVEfm 或 NVEfm。使用 NV 静电纺丝或 NV 和 PV 静电纺丝的组合可能非常有助于开发适合组织工程应用的合适支架。
