Ortu Silvia, Molicotti Paola, Sechi Leonardo Antonio, Pirina Pierp, Saba Franca, Vertuccio Cono, Deriu Antonella, Maida Ivana, Mura Maria Stella, Zanetti Stefania
Dip. di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli Studi di Sassari, Italy.
New Microbiol. 2006 Jan;29(1):75-80.
We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
我们开发了一种实时聚合酶链反应（PCR）检测方法，通过TaqMan检测法，利用iCycler iQ检测系统直接对临床标本进行检测，以检测结核分枝杆菌。总共从疑似结核病患者、患有活动性分枝杆菌感染的其他患者以及正在接受抗结核治疗的确诊结核病患者中采集了513份临床样本。将该检测方法的敏感性和特异性（分别为10%和100%）与传统微生物学方法的敏感性和特异性进行了比较。
