Perkins S L, Link D C, Kling S, Ley T J, Teitelbaum S L
Department of Pathology and Laboratory Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, Missouri 63110.
J Leukoc Biol. 1991 Nov;50(5):427-33. doi: 10.1002/jlb.50.5.427.
1,25-dihydroxyvitamin D3 [1,25(OH)2D] is known to stimulate maturation of the human promyelocytic line HL-60 and murine bone marrow precursor cells along a monocyte/macrophage pathway. In this study, the steroid's effects on the PLB-985 leukemic line were examined. We found that 1,25(OH)2D induces monocytic differentiation of PLB-985 as manifested by morphological appearance, histochemical staining, and changes in cell surface antigen expression. Additionally, there was acquisition of functional monocyte characteristics including phagocytic activity and superoxide anion production via the respiratory burst pathway. Steady state levels of mRNA derived from the leukocyte-specific proto-oncogene c-fgr were also increased by the steroid. Thus, 1,25(OH2)D effectively differentiates PLB-985 cells along a monocytic pathway, providing a useful model of macrophage differentiation.
已知1,25 - 二羟基维生素D3 [1,25(OH)2D]可刺激人早幼粒细胞系HL - 60和小鼠骨髓前体细胞沿单核细胞/巨噬细胞途径成熟。在本研究中，检测了该类固醇对PLB - 985白血病细胞系的作用。我们发现，1,25(OH)2D可诱导PLB - 985的单核细胞分化，这通过形态学外观、组织化学染色以及细胞表面抗原表达的变化得以体现。此外，还获得了功能性单核细胞特征，包括吞噬活性以及通过呼吸爆发途径产生超氧阴离子。该类固醇还使源自白细胞特异性原癌基因c - fgr的mRNA稳态水平升高。因此，1,25(OH)2D可有效地使PLB - 985细胞沿单核细胞途径分化，提供了一个有用的巨噬细胞分化模型。
