Katagiri K, Katagiri T, Koyama Y, Morikawa M, Yamamoto T, Yoshida T
Tokyo Institute for Immunopharmacology, Inc., Japan.
J Immunol. 1991 Jan 15;146(2):701-7.
It has been reported that src family protein-tyrosine kinases were expressed specifically in a certain lineage or differentiation stage of hematopoietic cells. To understand the molecular basis for differentiation and function of monocyte/macrophage, we investigated the expressions of src family genes by the HL-60 cells stimulated with differentiation-inducing agents. TPA and vitamin D3 (D3) were used as stimulants for monocytic development, since each agent has been known to induce phenotypically specific differentiation of HL-60 cells. The fyn, fgr, and lyn genes were characteristically expressed concomitantly with phenotypic changes and expressions of nuclear proto-oncogenes, whereas src, lck, hck, and yes genes were not. In TPA-induced differentiation of HL-60 cells, both fyn and lyn genes, but not fgr gene, were expressed. In contrast, both fgr and lyn genes, but not fyn gene, were expressed in D3-induced differentiation of the cells. The independent and characteristic expressions of these genes were observed in the further advanced differentiation of HL-60 cells induced by TPA plus D3 or D3 plus human transforming growth factor-beta 1. The granulocytic differentiation of the cells treated with retinoic acid was accompanied by intense expression of fgr, but weak or no expression of lyn and fyn gene. These data indicate that each protein-tyrosine kinase encoded by src family genes may play distinct roles in development and/or functions of monocyte/macrophage-lineage cells.
据报道，src家族蛋白酪氨酸激酶在造血细胞的特定谱系或分化阶段特异性表达。为了解单核细胞/巨噬细胞分化和功能的分子基础，我们研究了经分化诱导剂刺激的HL-60细胞中src家族基因的表达。由于已知TPA和维生素D3（D3）每种试剂均可诱导HL-60细胞发生表型特异性分化，因此将它们用作单核细胞发育的刺激剂。fyn、fgr和lyn基因的表达特征与表型变化以及核原癌基因的表达同时出现，而src、lck、hck和yes基因则不然。在TPA诱导的HL-60细胞分化中，fyn和lyn基因均表达，但fgr基因不表达。相反，在D3诱导的细胞分化中，fgr和lyn基因均表达，但fyn基因不表达。在TPA加D3或D3加人转化生长因子-β1诱导的HL-60细胞进一步分化过程中，观察到这些基因的独立和特征性表达。用视黄酸处理的细胞的粒细胞分化伴随着fgr的强烈表达，但lyn和fyn基因表达较弱或不表达。这些数据表明，src家族基因编码的每种蛋白酪氨酸激酶可能在单核细胞/巨噬细胞谱系细胞的发育和/或功能中发挥不同作用。
