Russo F D, Silhavy T J
Department of Molecular Biology, Princeton University, NJ 08544.
J Mol Biol. 1991 Dec 5;222(3):567-80. doi: 10.1016/0022-2836(91)90497-t.
Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a two-component regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species. OmpR-P.
细菌孔蛋白基因ompF和ompC的渗透调节由一个双组分调节系统控制。EnvZ是该系统的传感组分，能够对效应组分OmpR进行磷酸化和去磷酸化。在envZ中分离到了消除两种孔蛋白基因表达的突变体。这些突变体似乎丧失了EnvZ的激酶活性，但保留了其磷酸酶活性，因此在它们存在的情况下，OmpR完全未被磷酸化。这些突变体在单倍体以及与其他envZ等位基因的二倍体中的行为，与EnvZ通过控制单一物种OmpR-P的浓度来介导渗透调节的模型一致。
