一种来自双孢蘑菇新鲜子实体的新型核糖核酸酶。

A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus.

作者信息

Wang H X, Ng T B

机构信息

Department of Microbiology, China Agricultural University, Beijing and State Key Laboratory for Agrobiotechnology, China.

出版信息

Biochem Cell Biol. 2006 Apr;84(2):178-83. doi: 10.1139/o06-033.

DOI:10.1139/o06-033

PMID:16609698

Abstract

A 14 kDa ribonuclease with a novel N-terminal sequence was isolated from fresh fruiting bodies of the portabella mushroom. It was adsorbed on DEAE-cellulose and carboxymethyl-cellulose, and demonstrated the highest ribonucleolytic potency toward poly (A), 60% as much activity toward poly (C), 40% as much activity toward poly (U), and the least activity (7% as much) toward poly (G). It exhibited a pH optimum at pH 4.5 and a temperature optimum at 60 degrees C. Its activity at 100 degrees C was higher than that at 20 degrees C.

摘要

从新鲜的大褐菇子实体中分离出一种具有新型N端序列的14 kDa核糖核酸酶。它可吸附于DEAE - 纤维素和羧甲基纤维素上，对聚腺苷酸（poly (A)）表现出最高的核糖核酸水解活性，对聚胞苷酸（poly (C)）的活性为聚腺苷酸的60%，对聚尿苷酸（poly (U)）的活性为聚腺苷酸的40%，对聚鸟苷酸（poly (G)）的活性最低（为聚腺苷酸的7%）。其最适pH为4.5，最适温度为60℃。它在100℃时的活性高于20℃时的活性。