Dioguardi Nicola, Grizzi Fabio, Franceschini Barbara, Bossi Paola, Russo Carlo
Laboratori di Medicina Quantitativa, Istituto Clinico Humanitas IRCCS, Via Manzoni 56, 20089 Rozzano MI, Italy.
World J Gastroenterol. 2006 Apr 14;12(14):2187-94. doi: 10.3748/wjg.v12.i14.2187.
To provide the accurate alternative metrical means of monitoring the effects of new antiviral drugs on the reversal of newly formed collagen.
Digitized histological biopsy sections taken from 209 patients with chronic C virus hepatitis with different grade of fibrosis or cirrhosis, were measured by means of a new, rapid, user-friendly, fully computer-aided method based on the international system meter rectified using fractal principles.
The following were described: geometric perimeter, area and wrinkledness of fibrosis; the collation of the Knodell, Sheuer, Ishak and METAVIR scores with fractal-rectified metric measurements; the meaning of the physical composition of fibrosis in relation to the magnitude of collagen islets; the intra- and inter-biopsy sample variability of these parameters; the"staging" of biopsy sections indicating the pathway covered by fibrosis formation towards its maximum known value; the quantitative liver tissue architectural changes with the Hurst exponent.
Our model provides the first metrical evaluations of the geometric properties of fibrosis and the quantitative architectural changes of the liver tissue. The representativeness of histological sections of the whole liver is also discussed in the light of the results obtained with the Hurst coefficient.
提供准确的替代测量方法，以监测新型抗病毒药物对新形成胶原蛋白逆转的影响。
采用一种基于分形原理校正的国际单位制的新型、快速、用户友好的全计算机辅助方法，对209例不同纤维化或肝硬化程度的慢性丙型肝炎患者的数字化组织活检切片进行测量。
描述了以下内容：纤维化的几何周长、面积和皱缩度；Knodell、Sheuer、Ishak和METAVIR评分与分形校正测量值的对照；纤维化物理组成与胶原岛大小的关系；这些参数在活检样本内和样本间的变异性；活检切片的“分期”，表明纤维化形成向其最大已知值所覆盖的途径；用赫斯特指数表示的肝脏组织结构的定量变化。
我们的模型首次对纤维化的几何特性和肝组织的定量结构变化进行了测量评估。还根据赫斯特系数获得的结果讨论了全肝组织学切片的代表性。
