Cho Yong-Gu, Choi Byung-Jun, Song Jae-Whie, Kim Su-Young, Nam Suk-Woo, Lee Sug-Hyung, Yoo Nam-Jin, Lee Jung-Young, Park Won-Sang
Department of Pathology, College of Medicine, The Catholic University of Korea, Seocho-gu, Seoul 137-701, Korea.
World J Gastroenterol. 2006 Apr 14;12(14):2250-3. doi: 10.3748/wjg.v12.i14.2250.
To investigate whether krUppel-like factor 6 (KLF6) plays an important role in the development and/or progression of colorectal cancer.
A total of 123 formalin-fixed and paraffin-embedded colorectal cancer specimens were analyzed by immunohistochemistry using tissue microarray for the expression of KLF6 protein. The specimens were collected over a 3-year period in the laboratories at our large teaching hospital in Seoul, Republic of Korea. The correlation of KLF6 expression with clinicopathologic parameters was analyzed by chi2 test and Bartholomew test.
Normal colonic epithelium showed weak to moderate expression of KLF6, whereas reduced KLF 6 expression or loss of KLF6 expression was seen in 45 (36.6%) of the 123 colorectal carcinoma specimens. Interestingly, aberrant expression of KLF6 was detected in 25 (43.1%) of 58 cases with metastasis to regional lymph node and in 31 (47.0%) of 66 tumors more than 5 cm in size. Statistically, loss of KLF6 expression was significantly associated with tumor size (P < 0.05). However, there was no significant correlation between KLF6 expression and Dukes'stage (Bartholomew test, P > 0.05), tumor location and lymph node metastasis (chi2 test, P > 0.05).
Loss of KLF6 expression may be a common and early event in colorectal carcinogenesis.
研究Krüppel样因子6（KLF6）在结直肠癌的发生和/或发展过程中是否发挥重要作用。
采用组织芯片免疫组化法分析123例福尔马林固定石蜡包埋的结直肠癌标本中KLF6蛋白的表达情况。这些标本是在韩国首尔一家大型教学医院的实验室中，历时3年收集的。采用卡方检验和巴塞洛缪检验分析KLF6表达与临床病理参数的相关性。
正常结肠上皮显示KLF6呈弱至中度表达，而在123例结直肠癌标本中，有45例（36.6%）出现KLF6表达降低或缺失。有趣的是，在58例发生区域淋巴结转移的病例中，有25例（43.1%）检测到KLF6异常表达；在66例肿瘤大小超过5 cm的病例中，有31例（47.0%）检测到KLF6异常表达。统计学分析显示，KLF6表达缺失与肿瘤大小显著相关（P < 0.05）。然而，KLF6表达与Dukes分期（巴塞洛缪检验，P > 0.05）、肿瘤位置及淋巴结转移（卡方检验，P > 0.05）之间均无显著相关性。
KLF6表达缺失可能是结直肠癌发生过程中常见的早期事件。
