Kitchener H C, Neilson L, Burnett R A, Young L, Macnab J C
Department of Midwifery, University of Glasgow.
Br J Obstet Gynaecol. 1991 Oct;98(10):1042-8. doi: 10.1111/j.1471-0528.1991.tb15344.x.
To observe in serial fashion the histological changes of human papilloma virus (HPV) infection of the cervix and to correlate these with the detection of HPV genomes.
A prospective longitudinal study to perform colposcopy and at 0, 9 and 18 months to obtain a smear, a biopsy for histology and HPV-16 genome detection by DNA/DNA hybridization.
Glasgow Family Planning Centre.
Eighty-two women recruited on the basis of a routine smear showing viral change without dyskaryosis.
Women found to have CIN 3 were treated with laser.
Cytology and cervical biopsy results and HPV-16 DNA detection by Southern blotting obtained serially from individual subjects.
Of 82 women recruited, 10% were positive for HPV-16 detection by Southern blotting. Detection of HPV-16 genomes did not correlate with either CIN or viral infection assessed histologically and serial hybridizations in a given individual showed fluctuation in HPV-16 detection. Polymerase chain reaction in a subset of samples revealed 46% positive for HPV DNA.
No clear correlation exists between HPV genome detection and the histological appearance. HPV-16 genome detection alone may not be a useful predictor of precancerous progression.
以系列方式观察人乳头瘤病毒（HPV）宫颈感染的组织学变化，并将这些变化与HPV基因组检测结果相关联。
一项前瞻性纵向研究，进行阴道镜检查，并在0、9和18个月时获取涂片、组织学活检以及通过DNA/DNA杂交检测HPV-16基因组。
格拉斯哥计划生育中心。
82名基于常规涂片显示有病毒改变但无核异常的女性。
发现患有CIN 3的女性接受激光治疗。
从个体受试者连续获取的细胞学和宫颈活检结果以及通过Southern印迹法检测HPV-16 DNA。
在招募的82名女性中，通过Southern印迹法检测HPV-16呈阳性的占10%。HPV-16基因组的检测与组织学评估的CIN或病毒感染均无相关性，且对给定个体的系列杂交显示HPV-16检测存在波动。一部分样本的聚合酶链反应显示46%的HPV DNA呈阳性。
HPV基因组检测与组织学表现之间不存在明确的相关性。仅检测HPV-16基因组可能不是癌前病变进展的有用预测指标。
