Chernomordik L V, Papahadjopoulos D, Tsong T Y
Department of Biochemistry, University of Minnesota, St. Paul.
Biochim Biophys Acta. 1991 Nov 18;1070(1):193-7. doi: 10.1016/0005-2736(91)90163-3.
The influence of electric field treatments on the interaction of large unilamellar vesicles (liposomes) with animal cells was monitored by the fluorescence assay based on the use of the liposomes loaded by a dye 1-hydroxypyrene-1,3,6-trisulfonic acid (HPTS). It was shown that application of a short electric pulse (100 microseconds of 3-4 kV/cm) to the suspension of cells in presence of vesicles resulted in significant (more than 2 times) increase of the fluorescence associated with cells. The pH-sensitivity of the excitation spectrum of the dye and its interaction with the quencher were used to determine the nature of the phenomenon as the increase of the liposome binding onto the cell surface but not the consequence of a promotion of liposome uptake into the cells by endocytosis. The higher affinity for the liposome caused by the electric field has a lifetime of several minutes. The possible relation of the effect described to the electroporation of cell membranes and to macroscopic changes in membrane structure is discussed.
基于使用负载有染料1-羟基芘-1,3,6-三磺酸（HPTS）的脂质体的荧光测定法，监测了电场处理对大单层囊泡（脂质体）与动物细胞相互作用的影响。结果表明，在存在囊泡的情况下，对细胞悬液施加短电脉冲（3 - 4 kV/cm，100微秒）会导致与细胞相关的荧光显著增加（超过2倍）。利用染料激发光谱的pH敏感性及其与猝灭剂的相互作用来确定该现象的本质，即脂质体在细胞表面结合增加，而非通过内吞作用促进脂质体摄取到细胞内的结果。电场引起的对脂质体更高亲和力可持续几分钟。讨论了所描述的效应与细胞膜电穿孔以及膜结构宏观变化的可能关系。
