Atkins S R, Matteson E L, Myers J L, Ryu J H, Bongartz T
Division of Rheumatology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
Ann Rheum Dis. 2006 May;65(5):677-80. doi: 10.1136/ard.2005.043877.
The role of mast cells in extra-articular manifestations of rheumatoid arthritis (RA) has not been studied so far.
To characterise and quantify mast cells in RA associated interstitial pneumonia (IP) by an immunohistological study.
Lung biopsy specimens from 15 patients with RA associated IP, 12 patients with idiopathic IP, and 5 control patients were stained with antibodies directed against tryptase (mast cell marker). Morphological characterisation of stained specimens was carried out and staining was quantified by computer assisted image analysis.
Tryptase staining showed the marked presence of mast cells in idiopathic IP and in RA associated IP. A significant difference in stained tissue area was found between RA associated IP (2.6%, IQR 2.0-3.2%, p = 0.015) and idiopathic IP (3.1%, IQR 1.8-3.7%, p = 0.003) compared with control tissue specimens (1.0%, IQR 0.7-1.5%). The extent of mast cell infiltration correlated well and inversely with pulmonary function variables.
Mast cell infiltrates are present in RA associated IP and idiopathic IP. The observed correlation of pulmonary function and mast cell numbers would be consistent with the proposed role of mast cell mediators in the promotion of fibrogenesis. The findings provide a rationale for studying functional aspects of mast cell involvement in the pathogenesis of RA associated lung disease.
迄今为止，肥大细胞在类风湿关节炎（RA）关节外表现中的作用尚未得到研究。
通过免疫组织学研究对RA相关间质性肺炎（IP）中的肥大细胞进行特征描述和定量分析。
对15例RA相关IP患者、12例特发性IP患者和5例对照患者的肺活检标本用抗类胰蛋白酶（肥大细胞标志物）抗体进行染色。对染色标本进行形态学特征描述，并通过计算机辅助图像分析对染色进行定量。
类胰蛋白酶染色显示特发性IP和RA相关IP中均有大量肥大细胞存在。与对照组织标本（1.0%，四分位间距0.7 - 1.5%）相比，RA相关IP（2.6%，四分位间距2.0 - 3.2%，p = 0.015）和特发性IP（3.1%，四分位间距1.8 - 3.7%，p = 0.003）的染色组织面积存在显著差异。肥大细胞浸润程度与肺功能变量呈良好的负相关。
肥大细胞浸润存在于RA相关IP和特发性IP中。观察到的肺功能与肥大细胞数量的相关性与肥大细胞介质在促进纤维生成中所起的作用相符。这些发现为研究肥大细胞参与RA相关肺部疾病发病机制的功能方面提供了理论依据。
