Rossi M A, Curzio M, Di Mauro C, Fidale F, Garramone A, Esterbauer H, Torrielli M, Dianzani M U
Department of Experimental Medicine and Oncology, University of Turin, Torino, Italy.
Cell Biochem Funct. 1991 Jul;9(3):163-70. doi: 10.1002/cbf.290090304.
The effects of 4-hydroxy-2,3-trans-nonenal (HNE) and nonanal on the activity of phosphoinositide-specific phospholipase C of rat neutrophils have been studied in parallel with their action on neutrophil oriented migration. Concentrations of HNE ranging from 10(-7) to 10(-5) M significantly stimulated the oriented migration of rat polymorphonuclear leukocytes. HNE stimulated both the basal and GTP gamma S-induced phospholipase C activity when used at concentrations between 10(-8) and 10(-6) M. Nonanal was devoid both of chemotactic activity and of any action on phospholipase C activity. The effect of GTP gamma S on the stimulation of phospholipase C induced by HNE was higher when the lowest dose of the aldehyde was used; the finding of an additive effect between 10(-8) M HNE and 2 x 10(-5) M GTP gamma S suggests that the two compounds may share a final common pathway of action. These results suggest that the chemotactic activity of HNE might be mediated, like that of other more well-known chemoattractants, by the stimulation of phosphoinositide-specific phospholipase C.
研究了4-羟基-2,3-反式壬烯醛（HNE）和壬醛对大鼠中性粒细胞磷酸肌醇特异性磷脂酶C活性的影响，并将其与它们对中性粒细胞定向迁移的作用进行了平行研究。浓度范围为10^(-7)至10^(-5) M的HNE显著刺激了大鼠多形核白细胞的定向迁移。当以10^(-8)至10^(-6) M的浓度使用时，HNE刺激了基础磷脂酶C活性和GTPγS诱导的磷脂酶C活性。壬醛既没有趋化活性，也对磷脂酶C活性没有任何作用。当使用最低剂量的醛时，GTPγS对HNE诱导的磷脂酶C刺激的作用更高；在10^(-8) M HNE和2×10^(-5) M GTPγS之间发现的相加效应表明这两种化合物可能共享最终的共同作用途径。这些结果表明，HNE的趋化活性可能像其他更知名的趋化因子一样，通过刺激磷酸肌醇特异性磷脂酶C来介导。
