Rossi M A, Fidale F, Di Mauro C, Esterbauer H, Dianzani M U
Department of Experimental Medicine and Oncology, University of Turin, Italy.
Int J Tissue React. 1993;15(5):201-5.
Our work has evaluated the effects of certain lipid peroxidation products, i.e. 4-hydroxy-2,3-trans-nonenal (HNE), 4-hydroxy-2,3-trans-octenal (HOE), 2-trans-nonenal and nonanal, on phosphoinositide-specific phospholipase C (PL-C). The enzymatic activity has been determined in vitro by measuring the hydrolysis of labelled phosphatidylinositol-4,5-bisphosphate added to plasma membranes isolated from rat neutrophils. Concentrations of HNE between 10(-8) and 10(-6) M, and concentrations of HOE ranging from 10(-11) to 10(-8) M, were able to activate PL-C. Neither 2-trans-nonenal nor nonanal induced any change of PL-C activity. HNE, HOE and 2-nonenal, but not nonanal, have been shown to possess chemotactic properties towards rat neutrophils. The good correlation between the concentrations of HNE and HOE active on PL-C and those able to stimulate cell migration suggests that their chemotactic activity might be mediated by the activation of PL-C, as in the case of most chemoattractants. On the contrary, this mechanism of action cannot be applied to 2-nonenal, an aldehyde much more hydrophobic than HNE or HOE; its chemotactic activity might be the consequence of some perturbation of the lipidic environment of the cell membrane where it dissolves easily.
我们的研究评估了某些脂质过氧化产物，即4-羟基-2,3-反式壬烯醛（HNE）、4-羟基-2,3-反式辛烯醛（HOE）、反式-2-壬烯醛和壬醛，对磷酸肌醇特异性磷脂酶C（PL-C）的影响。通过测量添加到从大鼠中性粒细胞分离的质膜中的标记磷脂酰肌醇-4,5-二磷酸的水解，在体外测定酶活性。10^(-8)至10^(-6)M的HNE浓度以及10^(-11)至10^(-8)M的HOE浓度能够激活PL-C。反式-2-壬烯醛和壬醛均未引起PL-C活性的任何变化。已证明HNE、HOE和2-壬烯醛（而非壬醛）对大鼠中性粒细胞具有趋化特性。对PL-C有活性的HNE和HOE浓度与能够刺激细胞迁移的浓度之间的良好相关性表明，它们的趋化活性可能如大多数趋化因子一样，是由PL-C的激活介导的。相反，这种作用机制不适用于2-壬烯醛，它是一种比HNE或HOE疏水性更强的醛；其趋化活性可能是它容易溶解的细胞膜脂质环境受到某种扰动的结果。
