Enomoto M, Komoda Y, Tominaga A
Department of Biology, Faculty of Science, Okayama University, Japan.
Genetics. 1991 Nov;129(3):631-8. doi: 10.1093/genetics/129.3.631.
Strain 1485IN carries a chromosomal inversion which corresponds to 35% of the chromosome and includes proC, trp and his genes. The termini of the inversion lie between the lac and proC loci and between his and cdd of the normal strain. Using Tn10 and Tn5 in transduction crosses between the normal and inversion strains, the termini were mapped to sites located approximately 0.25 min and 1.6 min away from proC and his, respectively within a region of roughly 4 kb long. The crosses where the normal strains carrying Tn10 near the terminus are donors and the inversion strain is a recipient, yielded unusual Tetr His- recombinants, which arose from illegitimate recombination leading to the replacement of a chromosomal his+ region with a transducing fragment carrying proC. Another rearrangement was detected between the normal and inversion strains in a region outside the inverted segment near the cdd locus.
菌株1485IN携带一个染色体倒位，该倒位对应于染色体的35%，并包括proC、trp和his基因。倒位的末端位于正常菌株的lac和proC基因座之间以及his和cdd之间。在正常菌株和倒位菌株之间的转导杂交中使用Tn10和Tn5，末端被定位到分别距离proC和his约0.25分钟和1.6分钟的位点，位于一个大约4kb长的区域内。携带靠近末端的Tn10的正常菌株作为供体，倒位菌株作为受体的杂交产生了不寻常的Tetr His-重组体，这些重组体源于非法重组，导致携带proC的转导片段取代了染色体上的his+区域。在cdd基因座附近倒位片段之外的区域，在正常菌株和倒位菌株之间检测到另一种重排。
