大肠杆菌K-12中位于lac和cdd附近反向排列的IS3元件之间的大倒位1485IN
Large inversion in Escherichia coli K-12 1485IN between inversely oriented IS3 elements near lac and cdd.
作者信息
Komoda Y, Enomoto M, Tominaga A
机构信息
Department of Biology, Faculty of Science, Okayama University, Japan.
出版信息
Genetics. 1991 Nov;129(3):639-45. doi: 10.1093/genetics/129.3.639.
A companion study has shown that the inversion carried by strain 1485IN has one terminus between lac and proC and the other between his and cdd of the normal strain. Starting with this mapping data, we have done molecular work demonstrating that the inversion occurred by recombination between inversely oriented two IS3 elements, one present near lac and the other near the cdd locus; i.e., the inversion is IN(is3B-is3E). Evidence supporting this conclusion includes: (i) Normal and inversion strains share two short regions with identical restriction maps. One of these regions is near lac and the other near cdd. (ii) IS3 homology was detected in each of the terminus regions of both the normal and inversion strains. (iii) The sequence on one side of the original IS3 element near lac has been exchanged with the sequence on one side of the IS3 near cdd. Whether the inversion has occurred by one event of homologous recombination between the two IS3 elements or has been caused by involvement of IS3 elements on an F factor is discussed. Another rearrangement, probably related to inversion and deletion, was detected between the IS3 and cdd of the inversion strain.
一项配套研究表明，菌株1485IN携带的倒位中，一个末端位于正常菌株的lac和proC之间，另一个末端位于his和cdd之间。基于此定位数据，我们开展了分子研究，证明该倒位是由两个反向排列的IS3元件之间的重组所致，一个位于lac附近，另一个位于cdd位点附近；即，该倒位为IN(is3B-is3E)。支持这一结论的证据包括：(i) 正常菌株和倒位菌株共享两个具有相同限制性图谱的短区域。其中一个区域靠近lac，另一个区域靠近cdd。(ii) 在正常菌株和倒位菌株的每个末端区域均检测到IS3同源性。(iii) 原始IS3元件靠近lac一侧的序列已与靠近cdd的IS3一侧的序列发生交换。文中讨论了该倒位是由两个IS3元件之间的一次同源重组事件导致，还是由F因子上的IS3元件参与引起。在倒位菌株的IS3和cdd之间检测到另一种重排，可能与倒位和缺失有关。
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