Ginsburg M, MacLusky N J, Morris I D, Thomas P J
J Endocrinol. 1975 Mar;64(3):443-9. doi: 10.1677/joe.0.0640443.
High-affinity binding of [2,4,6,7-3-H]oestradiol-17 beta has been studied in cytosols prepared from hypothalami, pituitaries and uteri of female rats killed at different stages of the oestrous cycle, and in cytosols prepared from the hypothalami and pituitaries of male rats. In all cases the equilibrium dissociation constant of reaction was of the order of 10- minus 10 mol/1. The number of available high affinity sites per tissue (n) varied with physiological state. In females, n fluctuated with the oestrus cycle. In hypothalamus and pituitary, n fell by about 60 and 40% respectively in pro-oestrus, replenishment occurred during oestrus but could be delayed by phenobarbitone administration during the afternoon of pro-oestrus. In the uterus, n varied biphasically, there being peaks during dioestrus and oestrus, and troughs at pro-oestrus and metoestrus. The numbers of available sites at metoestrus were 12-5 times 10-9, 10-6 times 10-10 and 24-4 times times 10-10 for hypothalamus, pituitary and uterus respectively. In male rats, values for n were similar to those obtained for females at pro-oestrus (n/hypothalamus equals 6-8 times 10-9, n/pituitary equals 4-2 times 10-10). Binding was oestrogen specific in all the tissues studied.
已对处于发情周期不同阶段处死的雌性大鼠的下丘脑、垂体和子宫制备的胞质溶胶，以及雄性大鼠的下丘脑和垂体制备的胞质溶胶中[2,4,6,7-³H]雌二醇-17β的高亲和力结合进行了研究。在所有情况下，反应的平衡解离常数约为10⁻¹⁰mol/L。每个组织中可用的高亲和力位点数量（n）随生理状态而变化。在雌性中，n随发情周期波动。在下丘脑和垂体中，在发情前期n分别下降约60%和40%，在发情期有所补充，但在发情前期下午给予苯巴比妥可延迟补充。在子宫中，n呈双相变化，在间情期和发情期出现峰值，在发情前期和动情后期出现谷值。动情后期下丘脑、垂体和子宫的可用位点数量分别为12.5×10⁻⁹、10.6×10⁻¹⁰和24.4×10⁻¹⁰。在雄性大鼠中，n值与发情前期雌性大鼠的值相似（n/下丘脑 = 6.8×10⁻⁹，n/垂体 = 4.2×10⁻¹⁰）。在所研究的所有组织中，结合具有雌激素特异性。
