Kienker L J, Kuziel W A, Garni-Wagner B A, Kumar V, Tucker P W
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1991 Dec 15;147(12):4351-9.
The nature of TCR gamma and delta gene rearrangements in 4- to 6-week-old scid thymocytes was examined by using the polymerase chain reaction technique, cloning, and DNA sequencing. Analysis of 78 sequences indicates that TCR gamma and delta gene rearrangements in scid mice generally resemble those in thymocytes from normal young adult mice. V gamma 1, V gamma 2, and V gamma 5 rearrangements are heterogeneous, with extensive N region addition and nucleotide excision from the recombining coding segments. In addition, homogeneous and fetal-like V gamma 3, V gamma 4, and V delta 1 rearrangements are observed. These rearrangements are currently difficult to interpret but may be significant with respect to whether certain homogeneous joints in normal mice are due to cellular selection or to the rearrangement process. scid TCR gamma and delta gene nucleotide sequences also reveal direct V-J delta joining, inter-(V-J-C gamma) cluster joining, and the possibility of inversional rearrangement at the gamma locus. Short sequence homologies may contribute to V(D)J recombination and to the rescue of blocked coding joints.
利用聚合酶链反应技术、克隆和DNA测序，对4至6周龄重度联合免疫缺陷（scid）小鼠胸腺细胞中TCRγ和δ基因重排的性质进行了研究。对78个序列的分析表明，scid小鼠中的TCRγ和δ基因重排通常类似于正常成年小鼠胸腺细胞中的重排。Vγ1、Vγ2和Vγ5重排是异质性的，在重组编码片段中有广泛的N区添加和核苷酸切除。此外，还观察到了均一的、类似胎儿的Vγ3、Vγ4和Vδ1重排。目前这些重排难以解释，但对于正常小鼠中某些均一连接是由于细胞选择还是重排过程而言可能具有重要意义。scid TCRγ和δ基因核苷酸序列还揭示了直接的V-Jδ连接、（V-J-Cγ）簇间连接以及γ基因座处倒位重排的可能性。短序列同源性可能有助于V（D）J重组以及挽救受阻的编码连接。
