Taggart Edward W, Hill Harry R, Martins Thomas B, Litwin Christine M
Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.
Am J Clin Pathol. 2006 Mar;125(3):460-6.
We compared complement fixation (CF) for the measurement of antibodies against influenza A, influenza B, respiratory syncytial virus (RSV), human adenovirus, and parainfluenza viruses 1, 2, and 3 (para-1, para-2, and para-3) with 2 enzyme-linked immunosorbent assays (ELISA kits, A and B). The IgG ELISA kits compared very well with each other except for the influenza A and B IgG ELISAs. The IgG ELISAs, in general, did not agree with CF In contrast, the IgM ELISAs compared well with CF and each other except for the consensus parainfluenza panel from ELISA B. The poor agreement of the IgG ELISAs with the CF test can be explained by the increased sensitivity of the ELISAs and differences between CF antigens and the ELISA antigens. The influenza A and influenza B ELISA antigens differed between both kits, which may explain their poor agreement. The ELISA is a suitable replacement for CF, providing greater sensitivity, isotype specificity, and ease of use.
我们将补体结合试验（CF）与两种酶联免疫吸附测定法（ELISA试剂盒A和B）进行比较，以检测抗甲型流感病毒、乙型流感病毒、呼吸道合胞病毒（RSV）、人腺病毒以及1、2和3型副流感病毒（副流感病毒1、副流感病毒2和副流感病毒3）的抗体。除甲型和乙型流感病毒IgG ELISA外，两种IgG ELISA试剂盒之间的比较结果非常相似。总体而言，IgG ELISA与CF结果不一致。相比之下，除ELISA B的副流感病毒综合检测组外，IgM ELISA与CF以及它们彼此之间的比较结果都很好。IgG ELISA与CF检测结果一致性较差的原因可能是ELISA的敏感性增加以及CF抗原与ELISA抗原之间存在差异。两种试剂盒中甲型和乙型流感病毒ELISA抗原不同，这可能解释了它们一致性较差的原因。ELISA是CF的合适替代方法，具有更高的敏感性、同种型特异性且易于使用。
