Samoszuk M, Ravel J
Pathology Department, University of California, Irvine.
Lab Invest. 1991 Dec;65(6):631-6.
Epstein-Barr viral DNA (EBV DNA) has been detected in 20 to 58% of Hodgkin's disease tumors analyzed by Southern blotting or polymerase chain reaction (PCR). Because patients with Hodgkin's disease are generally immunodepressed, it is possible that the EBV is not directly involved in the pathogenesis of Hodgkin's disease but is merely detectable by molecular techniques because of reactivation of a latent infection. The purpose of this study was to determine if EBV DNA could be detected in an even higher percentage of cases of Hodgkin's disease, including acquired immunodeficiency syndrome (AIDS)-related Hodgkin's disease, by using newly designed, PCR amplification primers, and to compare the incidence of EBV DNA with the incidence of another common, latent virus (cytomegalovirus) in Hodgkin's disease tissue. The PCR was performed on DNA extracted from cells from 15 benign hyperplastic lymph nodes and from 15 cryopreserved cases of Hodgkin's disease, including 2 cases of AIDS-related Hodgkin's disease. For negative controls, PCR was also performed without template DNA and on genomic DNA from E. coli, calf thymus, a murine myeloma, and from a human cell line. After 32 cycles of amplification, a 225 base-pair amplification product comigrating with an EBV-positive control was detected in none of the negative controls but was present in 14 out of 15 cases (93%) of Hodgkin's disease, including both cases of AIDS-related Hodgkin's disease, and in 2 out of 15 cases of benign lymphoid hyperplasia. By contrast, cytomegalovirus DNA was undetectable by PCR in any of our specimens. We conclude that in our study set, the PCR procedure detected EBV-DNA but not cytomegalovirus DNA in a high percentage of cases of Hodgkin's disease, including two cases of AIDS-related Hodgkin's disease. These findings strengthen the hypothesis that EBV may be involved in the pathogenesis of Hodgkin's disease and AIDS-related Hodgkin's disease.
通过Southern印迹法或聚合酶链反应（PCR）分析发现，在20%至58%的霍奇金淋巴瘤肿瘤中可检测到爱泼斯坦-巴尔病毒DNA（EBV DNA）。由于霍奇金淋巴瘤患者通常存在免疫抑制，EBV可能并非直接参与霍奇金淋巴瘤的发病机制，而仅仅是由于潜伏感染的重新激活，通过分子技术才得以检测到。本研究的目的是确定使用新设计的PCR扩增引物，是否能在更高比例的霍奇金淋巴瘤病例中检测到EBV DNA，包括与获得性免疫缺陷综合征（AIDS）相关的霍奇金淋巴瘤，并比较EBV DNA在霍奇金淋巴瘤组织中的发生率与另一种常见潜伏病毒（巨细胞病毒）的发生率。对从15个良性增生性淋巴结以及15个冷冻保存的霍奇金淋巴瘤病例（包括2例与AIDS相关的霍奇金淋巴瘤）的细胞中提取的DNA进行PCR。作为阴性对照，还在无模板DNA的情况下以及对来自大肠杆菌、小牛胸腺、小鼠骨髓瘤和人细胞系的基因组DNA进行PCR。经过32个循环的扩增后，在所有阴性对照中均未检测到与EBV阳性对照共迁移的225个碱基对的扩增产物，但在15例霍奇金淋巴瘤病例中的14例（93%）中检测到，包括2例与AIDS相关的霍奇金淋巴瘤病例，在15例良性淋巴样增生病例中的2例中也检测到。相比之下，在我们所有的标本中均未通过PCR检测到巨细胞病毒DNA。我们得出结论，在我们的研究组中，PCR程序在高比例的霍奇金淋巴瘤病例（包括2例与AIDS相关的霍奇金淋巴瘤病例）中检测到了EBV-DNA，但未检测到巨细胞病毒DNA。这些发现强化了EBV可能参与霍奇金淋巴瘤和与AIDS相关的霍奇金淋巴瘤发病机制的假说。
