霍奇金淋巴瘤中EB病毒基因组的高发生率。
High incidence of Epstein-Barr virus genomes in Hodgkin's disease.
作者信息
Herbst H, Niedobitek G, Kneba M, Hummel M, Finn T, Anagnostopoulos I, Bergholz M, Krieger G, Stein H
机构信息
Institute of Pathology, Klinikum Steglitz, Free University of Berlin, West Germany.
出版信息
Am J Pathol. 1990 Jul;137(1):13-8.
Tissue specimens from 198 cases of Hodgkin's disease and 151 non-Hodgkin lymphomas, as well as 34 nonmalignant lymph node biopsies were examined for the presence of Epstein-Barr virus (EBV) DNA by polymerase chain reaction. Epstein-Barr virus-specific DNA sequences were detected in DNA extracts from frozen and/or paraffin-embedded tissues of 58% of Hodgkin's disease cases. High and low grade non-Hodgkin lymphomas as well as chronic lymphocytic leukemia biopsies contained EBV DNA in 26%, 14%, and 7% of the cases, respectively. Ten percent of the control lymph nodes with normal histology were EBV positive. In Hodgkin's disease biopsies, subsequent in situ hybridization revealed an exclusive localization of the viral DNA in the tumor cells. These findings suggest an involvement of EBV in the pathogenesis of Hodgkin's disease in a substantial proportion of cases.
通过聚合酶链反应检测了198例霍奇金病、151例非霍奇金淋巴瘤组织标本以及34例非恶性淋巴结活检标本中爱泼斯坦-巴尔病毒（EBV）DNA的存在情况。在58%的霍奇金病病例的冷冻和/或石蜡包埋组织的DNA提取物中检测到了爱泼斯坦-巴尔病毒特异性DNA序列。高级别和低级别非霍奇金淋巴瘤以及慢性淋巴细胞白血病活检标本中，分别有26%、14%和7%的病例含有EBV DNA。组织学正常的对照淋巴结中有10%为EBV阳性。在霍奇金病活检标本中，随后的原位杂交显示病毒DNA仅定位于肿瘤细胞中。这些发现提示在相当一部分病例中EBV参与了霍奇金病的发病机制。
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