Balakrishnan Gurusamy, Hu Ying, Spiro Thomas G
Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA.
Appl Spectrosc. 2006 Apr;60(4):347-51. doi: 10.1366/000370206776593799.
The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (approximately 10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing approximately 1 mJ NIR pulses that are tunable (1.80-2.05 microm) across the 1.9 microm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 degrees C, as measured via the O-H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.
本文描述了一种用于温度跃升（T-jump）应用的脉冲（10纳秒）可调谐近红外（NIR）激光源的工作特性。采用重复频率为1千赫兹的调Q Nd:YLF激光器（脉冲宽度约10纳秒）泵浦基于钛酸氧钾（KTA）晶体的光学参量振荡器（OPO），产生能量约为1毫焦的近红外脉冲，该脉冲在水的1.9微米振动倍频带范围内可调谐（1.80 - 2.05微米）。这个T-jump光源已与深紫外（UV）探测激光耦合，用于蛋白质动力学的拉曼研究。通过水的O - H伸缩拉曼带测量，很容易实现高达30摄氏度的温度跃升。文中展示了其在细胞色素c去折叠研究中的应用。
