Yakushijin Y, Yasukawa M, Kobayashi Y
First Department of Internal Medicine, School of Medicine, Ehime University.
Microbiol Immunol. 1991;35(8):655-60. doi: 10.1111/j.1348-0421.1991.tb01597.x.
The proliferative response of peripheral blood mononuclear cells (PBMC) from healthy adults to human herpesvirus-6 (HHV-6) was examined. It was found that PBMC from 13 of 14 HHV-6-seropositive adults apparently proliferated in response to stimulation with HHV-6 antigen in contrast to the lack of response of cord blood mononuclear cells. In order to confirm the presence of HHV-6-specific memory T cells in the peripheral blood of healthy adults, we established HHV-6-specific T-cell clones from an HHV-6-seropositive individual. CD4+ T-cell clones generated from HHV-6-stimulated PBMC were found to proliferate upon stimulation with HHV-6 in the presence of autologous antigen-presenting cells, but not in response to herpes simplex virus type 1 antigen or mock-infected control antigen. These results indicate that a T-cell immune response against HHV-6 infection is generally present in healthy adult populations.
研究了健康成年人外周血单个核细胞（PBMC）对人疱疹病毒6型（HHV - 6）的增殖反应。结果发现，14名HHV - 6血清阳性成年人中有13人的PBMC在HHV - 6抗原刺激下明显增殖，这与脐血单个核细胞缺乏反应形成对比。为了证实健康成年人外周血中存在HHV - 6特异性记忆T细胞，我们从一名HHV - 6血清阳性个体中建立了HHV - 6特异性T细胞克隆。发现从HHV - 6刺激的PBMC产生的CD4 + T细胞克隆在自体抗原呈递细胞存在的情况下，受到HHV - 6刺激时会增殖，但对1型单纯疱疹病毒抗原或模拟感染对照抗原无反应。这些结果表明，健康成年人群体中普遍存在针对HHV - 6感染的T细胞免疫反应。
