Han Xiao-Bing, Wang Fang, Yao Wei-Xing, Liu You-Qing, Wang Gang, Ma Ding
Medical Center of Tumor Biology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P. R. China.
Ai Zheng. 2006 Apr;25(4):451-5.
BACKGROUND & OBJECTIVE: Various potassium channels are known to be involved in proliferation of many malignant cell lines. This study was to explore the role of voltage-gated potassium channels in proliferation of human cervical carcinoma cells through observing the effects of tetraethylammonium (TEA) on proliferation and outward potassium currents in human cervical carcinoma cell line SiHa.
SiHa cells were treated with TEA. The effect of TEA on proliferation of SiHa cells was assessed by MTT assay. Cell apoptosis and cell cycle were detected by flow cytometry with Hoechst 33258 staining. The outward potassium currents were recorded by patch clamp technique.
TEA inhibited the proliferation of SiHa cells in dose-and time-dependent manners, and induced cell apoptosis. The cell cycle was arrested at G0/G1 phase after treatment with TEA. Exposure of SiHa cells to 10 mmol/L TEA reduced the peak outward potassium currents significantly from (260+/-12) pA to (58+/-6) pA (P<0.01).
Voltage-gated potassium channels play an important role in regulating proliferation of cervical carcinoma SiHa cells. Blocking voltage-gated potassium channels could inhibit proliferation of SiHa cells.
已知多种钾通道参与多种恶性细胞系的增殖。本研究旨在通过观察四乙铵（TEA）对人宫颈癌SiHa细胞系增殖和外向钾电流的影响，探讨电压门控钾通道在人宫颈癌细胞增殖中的作用。
用TEA处理SiHa细胞。采用MTT法评估TEA对SiHa细胞增殖的影响。用Hoechst 33258染色，通过流式细胞术检测细胞凋亡和细胞周期。采用膜片钳技术记录外向钾电流。
TEA以剂量和时间依赖性方式抑制SiHa细胞的增殖，并诱导细胞凋亡。用TEA处理后，细胞周期停滞在G0/G1期。将SiHa细胞暴露于10 mmol/L TEA后，外向钾电流峰值从（260±12）pA显著降低至（58±6）pA（P<0.01）。
电压门控钾通道在调节宫颈癌SiHa细胞增殖中起重要作用。阻断电压门控钾通道可抑制SiHa细胞的增殖。
