Aratake Yatsuki, Nomura Hajime, Kotani Tomio, Marutsuka Kosuke, Kobayashi Kaoru, Kuma Kanji, Miyauchi Akira, Okayama Akihiko, Okayama Akihiko
Central Laboratory for Clinical Investigation, Miyazaki University Hospital, Miyazaki, Japan.
Am J Clin Pathol. 2006 Mar;125(3):399-406. doi: 10.1309/LF3Q-1NQK-MT2N-9KNV.
The aim of the present study was to clarify the underlying molecules that might contribute to the highly aggressive behavior of anaplastic thyroid carcinoma. We selected 5 cases of anaplastic thyroid carcinoma that had a differentiated area to determine differences in the molecules of undifferentiated and differentiated cancer cells. We immunohistochemically examined the localization of nuclear antigen (Ki-67), proliferating cell nuclear antigen (PCNA), p53, apoptotic protease-activating factor-1 (Apaf-1), CD26, galectin-3, E-cadherin, and CD147. We found an increased Ki-67, PCNA, and p53 labeling indices; decreased levels of Apaf-1, CD26, galectin-3, and E-cadherin; and overexpression of CD147 in the undifferentiated area compared with the differentiated area. These findings indicate high proliferative properties, suppression of apoptosis, disruption of cell-cell interaction, and induction of matrix metalloproteinases in the undifferentiated areas. Thus the molecules examined might be useful for evaluating the aggressive nature of this tumor and the prognosis.
本研究的目的是阐明可能导致间变性甲状腺癌高度侵袭性行为的潜在分子。我们选取了5例具有分化区域的间变性甲状腺癌病例，以确定未分化癌细胞和分化癌细胞在分子水平上的差异。我们采用免疫组织化学方法检测了核抗原（Ki-67）、增殖细胞核抗原（PCNA）、p53、凋亡蛋白酶激活因子-1（Apaf-1）、CD26、半乳糖凝集素-3、E-钙黏蛋白和CD147的定位。我们发现，与分化区域相比，未分化区域的Ki-67、PCNA和p53标记指数增加；Apaf-1、CD26、半乳糖凝集素-3和E-钙黏蛋白水平降低；CD147过表达。这些发现表明，未分化区域具有高增殖特性、凋亡抑制、细胞间相互作用破坏以及基质金属蛋白酶的诱导。因此，所检测的分子可能有助于评估该肿瘤的侵袭性和预后。
