Domon Bruno, Aebersold Ruedi
Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, Switzerland.
Science. 2006 Apr 14;312(5771):212-7. doi: 10.1126/science.1124619.
Mass spectrometry is a central analytical technique for protein research and for the study of biomolecules in general. Driven by the need to identify, characterize, and quantify proteins at ever increasing sensitivity and in ever more complex samples, a wide range of new mass spectrometry-based analytical platforms and experimental strategies have emerged. Here we review recent advances in mass spectrometry instrumentation in the context of current and emerging research strategies in protein science.
质谱分析是蛋白质研究以及一般生物分子研究的核心分析技术。在对蛋白质进行日益灵敏的鉴定、表征和定量分析以及处理日益复杂的样本的需求推动下，出现了一系列基于质谱分析的新型分析平台和实验策略。在此，我们结合蛋白质科学当前及新出现的研究策略，综述质谱分析仪器的最新进展。
