脂质体包裹的内消旋-2,3-二巯基琥珀酸对预先接触镉的小鼠体内镉的动员作用。

Mobilization of cadmium by liposome-encapsulated meso-2,3-dimercaptosuccinic acid in pre-exposed mice.

作者信息

Srivastava S, Gupta S, Behari J R, Srivastava R C

机构信息

Industrial Toxicology Research Centre, Lucknow, India.

出版信息

Toxicol Lett. 1991 Dec;59(1-3):125-31. doi: 10.1016/0378-4274(91)90063-c.

DOI:10.1016/0378-4274(91)90063-c

PMID:1661448

Abstract

meso-2,3-Dimercaptosuccinic acid (DMSA) treatment in free of liposome-encapsulated form was given to mice pre-exposed to cadmium as CdCl2 (2 intraperitoneal injections; 0.5 mg Cd/kg along with 5 microCi 109CdCl2 in 4 ml volume within 24 h). Both treatments removed cadmium from liver, spleen, testis and blood with liposomal DMSA exhibiting higher efficacy in mobilizing cadmium not only from whole organs but also from liver proteins. It also resulted in higher excretion of cadmium via urine as compared with free DMSA or saline treatment. Whereas this treatment eliminated significantly higher amounts of cadmium via the fecal route throughout the period examined, free DMSA responded only 48 h after treatment and was less effective. The results suggest mobilization of cadmium from intracellular sites of deposition. However, DMSA in the dose administered (24 mumol/kg i.v.) in either form was ineffective in decorporating cadmium from the kidney, the critical organ in cadmium intoxication.

摘要

将游离形式（无脂质体包裹）的内消旋 - 2,3 - 二巯基丁二酸（DMSA）给予预先经氯化镉（CdCl₂）染镉的小鼠（腹腔注射2次；24小时内分两次腹腔注射，每次0.5 mg Cd/kg，同时在4 ml溶液中加入5 μCi¹⁰⁹CdCl₂）。两种处理方式均能使肝脏、脾脏、睾丸和血液中的镉排出，脂质体包裹的DMSA不仅在从整个器官中动员镉方面，而且在从肝脏蛋白质中动员镉方面都表现出更高的效能。与游离DMSA或生理盐水处理相比，它还导致通过尿液排出更多的镉。虽然在整个检查期间，这种处理方式通过粪便途径排出的镉量显著更高，但游离DMSA仅在处理后48小时才有反应且效果较差。结果表明镉从细胞内沉积部位被动员出来。然而，无论哪种形式，所给予剂量（静脉注射24 μmol/kg）的DMSA对镉中毒的关键器官——肾脏中的镉促排均无效。